Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2748

Full Length Research Paper

Formulation, characterization and ex-vivo permeation studies on gentamicin-loaded transdermal patches based on PURASORB® polymers

  Petra Obioma Nnamani1, Franklin Chimaobi Kenechukwu1*, Esther Uju Dibua2, Celestine Chidi Ogbonna2, 3, Mumuni Abdul Momoh1, Augustina Uche Olisemeka1, Agubata Chukwuma Obumneme4 and Anthony Amaechi Attama1
    1Drug Delivery Research Unit, Department of Pharmaceutics, University of Nigeria, Nsukka 410001,Enugu State, Nigeria. 2Department of Microbiology, University of Nigeria, Nsukka 410001, Enugu State, Nigeria. 3Microbiology Unit, School of Bioscience and Biotechnology, University of Camerino, 62032 Camerino, Italy. 4Department of Pharmaceutical Technology and Industrial Pharmacy, University of Nigeria, Nsukka 410001, Enugu State, Nigeria.  
Email: [email protected], [email protected]

  •  Accepted: 12 April 2013
  •  Published: 30 June 2013

Abstract

 

 

Topical administration of gentamicin, an aminoglycoside antibiotic commonly used for treatment of bacterial infections, is limited by toxicity and membrane impermeability. The purpose of this study was to develop an alternative non-invasive, convenient and cost-effective drug delivery system for enhanced skin delivery of gentamicin. The patches were formulated by solvent evaporation technique using PURASORB® polymers and evaluated for drug content, thermal properties, physicochemical performance, stability, skin irritability and ex-vivo drug permeation through rat skin using a modified Franz diffusion cell. The DSC results indicate absence of strong interaction between gentamicin and the polymers. Theformulations showed good drug encapsulation, stability, physicochemical properties, tolerability on rat skin and ex-vivo drug permeation through rat skin.Compared with a commercially available gentamicin sulphate cream the transdermal patches gave higher ex-vivo skin permeation through rat skin with patches of PURASORB® PL 32 showing highest permeation flux (5.161 µg/cm2.h) and permeation coefficient (1.032 × 10-6 cm/h). The results of this study indicates that patches of PURASORB® PL 32 represent a new delivery system for enhnaced skin delivery of gentamicin.

 

Key words: Transdermal patches, gentamicin, PURASORB® polymers,bioadhesive strength, ex-vivo drug release.

 

Abbreviation

Abbreviations: TDDS, Transermal drug delivery systems; PL 32, PDL 04, PDL 05 and PLGA are gentamicin-loaded patches containing PURASORB® PL 32, PDL 04, PDL 05 and PLGA respectively; APIs, active pharmaceutical ingredients; IZD,inhibition zone diameter; DSC, differential scanning calorimetry.