Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2767

In vitro evaluation of plant extracts against Alternaria brassicae (Berk.) Sacc. causing leaf spot of mustard and Fusarium oxysporum f. sp. lycopersici causing wilt of tomato

S. A. Ganie1*, V. R. Pant1, M. Y. Ghani2, Ashiq Hussain Lone3, Qaisar Anjum4and S. M. Razvi5    
1Department of Plant Protection, Allahabad Agricultural Institute – Deemed University- Allahabad- 211007, India. 2Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar – 191 121, India. 3Department of Botany, University of Kashmir, Srinagar, India. 4Centre for Forest Management Studies, School of Environmental Biology, Awdesh Pratab Singh University, Rewa, M.P, India. 5Division of Plant Breeding and Genetics, SKUAST-K Shalimar Kashmir, Srinagar, India.  
Email: [email protected]

  •  Accepted: 23 September 2013
  •  Published: 04 October 2013


Extracts of five plants viz. Azadirachta indica, Lantana camara, Ocimum sanctum, Eucalyptus globulus and Calotropis gigantea were evaluated in vitro by poison food technique (PFT) @ 3, 5, 7 and 9% concentrations against Alternaria brassicae andFusarium oxysporum f. sp. lycopersici causing blight of mustard and wilt of tomato respectively. In vitro study on A. brassicae revealed that all five plant extracts at all four concentrations significantly inhibited the mycelial growth of this pathogen as compared to control. However O. sanctum was found most efficacious with growth inhibition of (31.85%) followed by E. globulus (28.97%) and L. camara (23.60%). While in in vitro study of F. oxysporum f. sp. lycopersici A. indica extract was found most efficacious with growth inhibition of (29.33%) followed by E. globulus extract (28.72%). Therefore more evaluations in the green house of field experiments may validate their antifungal activity.


Key words: Alternaria brassicae, plant extracts, poison food technique, Fusarium oxysporum, tomato.