Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2755

Full Length Research Paper

Plasmid-mediated antibiotic resistance in Staphylococcus aureus from patients and non patients

Daini O. A.1* and Akano S. A.2
  1Department of Biochemistry Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Remo Campus, P. M. B. 2005, Ikenne, Ogun State, Nigeria. 2Department of Medical Microbiology and Parasitology, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Remo Campus, P. M. B. 2005, Ikenne, Ogun State, Nigeria.
Email: [email protected]

  •  Accepted: 06 February 2009
  •  Published: 30 April 2009

Abstract

 

Out of 156 samples collected from non-patients, nose and ear, 32% were found to harbor Staphylococcus aureus. Also 26 hospitals strains of S. aureus were isolated from clinical specimens. 62% of the total isolates including the clinical isolates were found to be multiple resistant, being resistant to 4 or more types of antibiotics. Resistance to methicillin and co-trimoxazole were the highest (76%) while resistance to gentamycin and erythromycin were the lowest (32%). Of the 74 strains, 62% were found to exhibit multiple resistant to 4 or more antimicrobial agents tested. 47% of the antibiotic resistant strains harboured plasmids ranging in molecular sizes from 0.282 kb and grouped into 6 plasmid profiles. Transformation experiment revealed that 41.2% of the resistant strains carried resistant plasmids of sizes 1.26, 23.13 and 25.12 kb. Plasmid-mediated resistance to amoxycillin, tetracycline and chloramphenicol was found.

 

Key words: Plasmids, Staphylococcus aureus, antibiotics, resistance.