Full Length Research Paper
This research was conducted to produce doubled haploid lines of wheat through chromosome elimination (wheat × maize crosses) technique. Three wheat hybrids: DH-29: MILAN / SH7 // SHIROODI, DH-30: INIA // SW89.3064 / STAR / 3 / MILAN / SHA7 and DH-32: NANJING8201 / KAUZ // MILAN / SHA7 were pollinated with maize pollen from two genotypes H1: KSC108, 403 and combination of pollen of them, to produce doubled haploid lines. Fertilization frequencies ranged from 87.35% in H1 genotype of maize to 92.96% in 403 genotype of maize (mean 90.15%) was significantly different among the maize genotypes. Embryo formation in the dissected seeds ranged from 93.59% in DH-29 genotype of wheat to 27.18% in DH-32 genotype. Out of the 517 cultured embryos (mean 27.18%), 80.69% germinated and 69.93% of them developed into plants. After colchicines treatment, 53.06% of plants survived and their seeds were harvested. Significant variation was found among wheat and maize progenies in the frequency of haploid plants. Analysis of variance showed significant differences among genotypes, for all traits. Overall, these results suggest that the doubled haploid system could be used as an effective means to produce desirable resistant lines in the shortest time.
Key words: Wheat × maize cross, doubled haploids, yellow rust resistance.
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