Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Expression of MUC2 and MUC5B in ethmoid sinus mucosa of patients with chronic rhinosinusitis

  Huang Xue-Kun*, Li Yuan, Ye Jin, Li Peng and Liu Hong    
Department of Otolaryngology-Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-Sun University, Guangzhou 510630, China.
Email: [email protected]

  •  Accepted: 03 June 2010
  •  Published: 04 July 2010

Abstract

This study investigated the expression of MUC2 and MUC5B in the ethmoid sinus mucosa of patients with chronic rhinosinusitis (CRS). Immunohistochemistry and fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR) were performed to detect the expression of MUC2 and MUC5B in the ethmoid sinus mucosa of 32 patients with CRS and 7 control individuals. Immunohistochemistry confirmed the expression of MUC2 and MUC5B in the ethmoid sinus mucosa of both patients and control subjects, and the expression of both proteins in CRS patients was significantly higher than that in control individuals (p < 0.01). The mRNA expression of MUC2 and MUC5B was 10.55 ± 3.91 × 104 and 18.80 ± 15.68 × 10copies/μg in CRS patients, and 1.36 ± 0.46 × 104 copies/μg and 1.49 ± 0.46 × 105 copies/μg in control individuals, respectively, with significant differences between the two groups(p < 0.01). Statistical analysis demonstrated the markedly increased expression of MUC2 and MUC5B in the ethmoid sinus mucosa of CRS patients, when compared with control individuals. The expression MUC2 and MUC5B in the ethmoid sinus mucosa of CRS patients was up-regulated, and the up-regulated expression may result in mucus hypersecretion and compromised mucociliary clearance in CRS patients.

 

Key words: chronic rhinosinusitis, mucin, immunohistochemistry, fluorescence quantitative polymerase chain reaction.