Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2730

Full Length Research Paper

Aberrant expression of monocyte chemoattractant protein-1 (mcp-1) in interstitial cystitis patients

  Jianwei Lv1, Yi Luo2, Jing Leng1, Wei Xue1, Dongming Liu1and Yiran Huang1*        
  1Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China. 2Department of Urology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA.
Email: [email protected]

  • Article Number - A0E580816300
  • Vol.5(7), pp. 663-667, April 2010
  •  Accepted: 17 February 2010
  •  Published: 30 April 2010

Abstract

 

Our present study investigated monocyte chemoattractant protein-1 (MCP-1) expression in bladder tissue and urine from interstitial cystitis (IC) patients. Totally 35 patients with IC, 20 patients with urinary tract infection (UTI) and 25 healthy subjects were recruited into this present study. IC was diagnosed according to the NIDDK IC Diagnosis Standard. All IC patients received 24 h voiding diaries, O’Leary-Sant IC questionnaires, potassium sensitivity test (PST) and cystoscopy. RT-PCR and ELISA were used to determine the levels of MCP-1 in bladder tissues and urine specimens of IC patients, UTI patients and healthy controls. Immunohistochemistry staining was used to determine the MCP-1 distribution in bladder tissue. ELISA results showed that urinary MCP-1 was 74.1 ± 36.9 pg/ml and 280.6 ± 68.9 pg/ml in IC and UTI patientsand10.8 ± 6.9 pg/ml in healthy controls (p < 0.01), respectively. RT-PCR analysis also revealed a moderate expression of MCP-1 mRNA in bladder tissue of IC patients. The severity of IC clinical symptoms was correlated with the levels of MCP-1. IC patients exhibited increased levels of MCP-1 in urine and bladder tissue. Increased MCP-1 may suggest IC after exclusion of UTI.

 

Key words: Interstitial cystitis, monocyte chemoattractant protein-1, urinary infection.

Abbreviation

 

IC, Interstitial cystitis; UTI, urinary tract infection PST, potassium sensitivity test; PUF, pelvic pain urgency/ 
frequency; APF, antiproliferative factor; PCR, polymerase chain reaction; EGF, epidermal growth factor.  

  • Articles on Google by: