Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2752

Full Length Research Paper

The effects of hydromethanolic extract of saffron (Crocus sativus) on some biochemical parameters of serum blood in constant darkness and light conditions in healthy male rats

  Ali Arasteh1*, Ali Aliyev2, Saeed Khamnei3, Abbas Delazar4, Mehran Mesgari4, Yousef Mehmannavaz5 and Maliheh Hamzehzadeh Azar6    
  1Department of Physiology, Islamic Azad University, Maragheh Branch, Maragheh, Iran. 2Department of Physiology, Faculty of Biology, Baku State University, Azerbaijan. 3Department of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran. 4School of Pharmacy, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 5Department of Animal Science, Islamic Azad University, Maragheh Branch, Maragheh, Iran. 6Department of Nursing, Islamic Azad University, Maragheh Branch, Maragheh, Iran.  
Email: [email protected]

  •  Accepted: 20 September 2011
  •  Published: 09 November 2011

Abstract

 

The purpose of the present investigation was to determine the effects of hydromethanolic extract of saffron on changes of serum cholesterol, glucose and insulin levels in constant darkness and light conditions in healthy male rats. In this study, healthy male rats (n = 30) were divided into 3 groups of tens and kept at various light/dark conditions: Control + saffron 12:12 light/dark (LDS); constant darkness + saffron (DDS) and constant light + saffron (LLS) groups for 2 weeks. All the three groups received 50 mg/kg saffron extract through intraperitoneal injection for 2 weeks. Daily injection was repeated at 10 am. Blood samples were obtained from retro-orbital sinus before administration and on the 7 and 14th days of administration. The serum cholesterol and glucose levels were measured by the enzymatic method and insulin levels were measured using insulin kit by enzyme-linked immunosorbent assay (ELISA) method. The results showed that the serum cholesterol levels of all three groups decreased after 2 weeks of experiment and the most reduction was seen in DDS group. Comparison of serum insulin in all groups did not show any significant changes, but serum glucose level in LLS group significantly increased compared with the two other groups (P<0.05). In contrast, the levels of serum glucose significantly decreased in DDS and LDS groups compared to LLS group (P<0.05). In conclusion, these results clearly indicated that saffron extract and constant darkness have hypoglycemic and hypolipidemic effects, but constant light condition showed hyperglycemic effect on serum blood in healthy male rats.

 

Key words: Saffron, constant darkness, light, insulin, cholesterol, glucose, healthy male rat.