Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2732

Full Length Research Paper

A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions

Paula Alejandra Sacca1*, Virginia Pistone Creydt1*, Amelia Julieta Tesone1 and Juan Carlos Calvo1,2
  1Laboratorio de Proteoglicanos y Matriz Extracelular, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. 2Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Email: [email protected]

  • Article Number - E7782C819974
  • Vol.4(12), pp. 1487-1495, December 2009
  •  Accepted: 27 October 2009
  •  Published: 31 December 2009

Abstract

 

The goal of the present work was to obtain a simple and reproducible experimental model that would maintain the characteristics of the extracellular physiological environment of breast epithelial cells, both in factors as well as stromal structure, on which we could grow and evaluate changes of normal and tumor breast cells. 3T3-L1 pre-adipocytes (breast stromal cell model) were cultured and irradiated in a microwave oven at different times and potencies. In order to lose their proliferation ability, cells had to be irradiated twice at 650 Watts with a two-minute pulse each. The characteristics of the treated stromal support were analyzed for cell morphology, presence of DNA and proteins. We then evaluated on this support the effect on proliferation and migration of both normal and tumor - murine and human - breast epithelial cells. Both cell types increased their proliferation, while only tumor cells increased migration, thus improving their metastatic capacity. We believe this is a new and simple experimental method of studying epithelial-stromal cell interaction.

 

Key words: Cancer, epithelial-stromal interactions, extracellular matrix, microwave oven, tumor epithelial cells.

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