This study aims to investigate the effect of ultra-short wave on hormone-induced ischemic necrosis of femoral head in early stage by using an Avascular necrosis of the femoral head (ANFH) rabbit model. Horse serum was administered to male rabbits via the marginal ear vein, whereas control rabbits received saline. After five weeks, the rabbits with horse serum were injected with methylprednisolone. Blood-lipoids and hemodynamic parameters were analyzed. Collagen type I was analyzed by using immunohistochemistry. Pathological examination of femoral head was analyzed by hematoxylin-eosin (HE) staining; Expression of vascular endothelial growth factor (VEGF) and BMP-2 mRNA was analyzed by RT-PCR. Results revealed that when compared with control group, the difference in model group was significant (P<0.05 or P<0.01), with regard to TXB2, 6-keto-PGF1α, blood fat level, T/P, and hemodynamic parameters, while compared with model group, the difference of USW group was significant (P<0.05 or P<0.01). The expression of collagen type I was significantly lower in the model animals than that in the controls, while that of the USW diathermy group was significantly higher than the control group (P<0.05). Pathology index was significantly different in the USW diathermy animals than that in the controls (P<0.05). Real-time PCR showed that VEGF mRNA and BMP-2 mRNA levels in the model group were higher signiï¬cantly as compared to those in the control group (P<0.01), while that in the diathermy group were signiï¬cantly higher than those in the model group (P<0.01). The study thus revealed that ultra-short wave has a positive curative effect on ANFH.
Key words: Ultra-short wave, Avascular necrosis of the femoral head (ANFH), Collagen Type I, VEGF mRNA, BMP-2 mRNA.