Abstract

Angiogenesis is a complex activity needed for physiological process. However, it is required for many pathological conditions as solid tumor progression and metastasis. So, today, researchers study on angiogenesis to find new clinical methods. In this research, Ross fertilized eggs were used and were divided in 5 random groups of 70 which consisted of : control, sham-exposed, treated with 5 μl sodium valproate solution, treated with 0.04 T electromagnetic field and treated with both sodium valproate and 0.04 T electromagnetic field. On day 12, chorioallantoic membrane (CAM) was examined and photographed. Then the numbers and lengths of vessels in special area on CAM were measured and compared with each other by t-test (p < 0.05). Comparison between average number and length of vessels in controls and sham-exposed did not show any significant difference. In groups 3 and 4, a significant decrease was shown in comparison with controls. Comparison between group 3 and 5 showed a significant decrease in the average number and length of vessels in group 5. As a result, we found that 0.04 T magnetic field could enhance the effect of sodium valproate as an anti-angiogenic drug on CAM.

Key words: Angiogenesis, chorioalantoic membrane, sodium valproate, magnetic field.