Abstract

Coleus tuberosus (Blume) Benth. is classified as a local vegetable of the familyLamiaceae, subfamily Nepetoideae and tribe Ocimeae. Ethanolic extract the Flesh of C. tuberosus and ethanolic extract of the peel of C. tuberosus evaluated antioxidant properties based on 1,1-Diphenyl-2-picryl hydrazyl radical assay, antiproliferative based on 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide assay and morphological change apoptosis based on staining with acridine orange and ethidium bromide using human breast cancer Michigan Cancer Foundation 7 (MCF 7) cells. Results showed that antioxidant and antiproliferative activity of ethanolic extract of the peel of C. tuberosus was higher than ethanolic extract of the flesh of C. tuberosus. Based on morphological changes with acridine orange and ethidium bromide staining, ethanolic extract of the flesh of C. tuberosus, ethanolic extract of the peel of C. tuberosus, ursolic acid and oleanolic acid have antiproliferative activity through induced apoptosis in MCF-7 cells. The antioxidant and antiproliferative activities were in a dose-dependent manner. The antioxidant activities and antiproliferative activity of ethanolic extract of the the flesh of C. tuberosus and ethanolic extract of the peel of C. tuberosus related with oleanolic acid and ursolic acid content. These result indicated that ethanolic extract of the flesh of C. tuberosus and ethanolic extract of the peel of C. tuberosus might be used as a potential source of natural antioxidants and antiproliferative agents.

Key words: Antioxidant, antiproliferative, apoptosis, Coleus tuberosus, ursolic acid, oleanolic acid.