Abstract

Rosa chinensis Jacq. is used worldwide in ornamental and medicine. The goal of this study was to establish a convenient media for plant regeneration and embryogenesis from nodal segments excised from greenhouse-grown plants and cultured on MS (200 mM NaCl-free/not) medium. Salinity stress could cause embryo death, plantlet morphological change and drastic necrosis during rooting and pot soil transplantation. The most effective embryo development (Dv), proliferation (Pl) and differentiation (Df) media under salt stress were respectively supplemented with [2.00 mg/L zeatin, 0.30 mg/L α-napthleneacetic acid (NAA), 1.50 mg/L gibberellic acid (GA₃)]; [5.00 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D), 0.50 mg/L zeatin, 1.50 mg/L GA³] and [2.00 mg/L zeatin, 1.50 mg/L GA₃, 3.00 mg/L abscisic acid (ABA)]. These media provided an average of 100±0.00, 88.35±0.68 and 51.59±1.78% respectively as relatively high percentage of well developed embryos. Analysis of macromolecules nitrogen (N), phosphorus (P) and potassium (K) of 200 mM NaCl stress in vitro (SSIV) were significantly higher compare to control and 200 mM NaCl-free recovered in vitro (RIV) plantlets. Chlorophyll in contrary was significantly higher in control and RIV compare to SSIV plantlets. Interestingly, Water and proline remained significantly higher in RIV (371.42±0.88 µg/g and 69.24±0.65% respectively) compare to control (368.79±0.58 µg/g and 54.67±1.31% respectively) and thus, China rose was improved of physiological activity.

Key words: Embryogenesis, plant regeneration, Rosa chinensis Jacq. ,transplantation, salinity stress.