In current study using Saponaria officinalis (Related to Caryophyllaceae family) hydroalcoholic extracts for in vitro growth inhibition of an avian isolated fatal Escherichia coli (isolated from coli CRD-complex carcasses) were reported. The disc diffusion method was used for evaluation of the antibacterial effects of Saponaria extracts in comparison to some routine antibiotics. Minimum inhibitory concentration (MIC) of Saponaria extracts against avian pathogenic E. coli, APEC, using tube dilution technique was studied. In titration technique for MIC, the concentration of the extracts was estimated in v/v in Dimethyle sulfoxide (DMSO) as inert solvent. For minimum bacterial concentration (MBC) studies 0.1 ml of MIC titer beside, a tube before and after, MIC titer were used for cultivation on Muller Hinton agar. Based on the results, the zones of inhibition for 10, 15 and 20 µl of Saponaria disks were 16, 18 and 19 mm, respectively, and the zones of inhibition diameter for antibiotic disks of Fosbac (Fos), Oxytetracycline (Oxy) and Danofloxacin (Dfx) were 15, 0 and 14 mm, respectively. In MIC technique, it is oriented that the concentration of 1/80 inhibits APEC growth but for MBC, the bacterial growth on Muller Hinton agar was not stopped. The present study has revealed that saponin extract from S. officinalis has useful antibacterial effects.
Key words: Antibacterial effects, coli chronic respiratory disease (CRD), Escherichia coli, minimum bacterial concentration (MBC), minimum inhibitory concentration (MIC), Saponaria officinalis, Saponin.
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