African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Lignin content in seed coats of Glyphosate-resistant soybean and their respective parents

Edicléia Aparecida Bonini
  • Edicléia Aparecida Bonini
  • Department of Biology, Federal Technological University of Parana, Zip code: 85892-000, Santa Helena, Paraná, Brazil.
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Patricia da costa Zonetti
  • Patricia da costa Zonetti
  • Department of Agronomy, Z Federal University of Paraná, ip code: 85950-000, Palotina, Paraná, B Brazil.
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Ana Paula Ferro
  • Ana Paula Ferro
  • Department of Biochemistry, University of Maringá, Zip code: 87020-900, Maringá, PR, Brazil.
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Osvaldo Ferrarese- Filho
  • Osvaldo Ferrarese- Filho
  • Department of Biochemistry, University of Maringá, Zip code: 87020-900, Maringá, PR, Brazil.
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Maria de Lourdes Lucio Ferrarese
  • Maria de Lourdes Lucio Ferrarese
  • Department of Biochemistry, University of Maringá, Zip code: 87020-900, Maringá, PR, Brazil.
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  •  Received: 31 August 2015
  •  Accepted: 20 November 2015
  •  Published: 14 July 2016

Abstract

The large-scale cultivation of Roundup Ready (RR) has recently increased among Brazilian farmers. However, few studies have compared the intrinsic characteristics of the seeds of RR soybean cultivars and their respective conventional parental. Thus, the purpose of this study is to verify if the genetic modification of the RR soybean affects the lignin content and its monomeric composition in the seed coats, in comparison with the parental cultivar. To do that, five groups, each one with a RR cultivar and its respective parental were selected. After the physiological analysis of the seeds, the coats were separated and dried in an incubator at 80°C for 24 h. Next, the contents of lignin and its monomers p-hydroxyphenyl (H), guaiacyl (G) and syringyl (S) were determined. In order to compare the RR cultivars and their respective parents, a statistical evaluation of contrasts was performed. The results revealed that only the group BRS133 vs BRS245RR was different; the transgenic cultivar showed significant increases in the lignin contents and their monomers. In conclusion, the introduction of the sequence CP4-EPSPS in the genome of soybean cultivars, had no any influence on seed coat lignification.

Key words: Monolignols, seeds, Glycine max L. Merrill, RoundupâReady.