African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6654

Full Length Research Paper

Lignin content in seed coats of Glyphosate-resistant soybean and their respective parents

Edicléia Aparecida Bonini
  • Edicléia Aparecida Bonini
  • Department of Biology, Federal Technological University of Parana, Zip code: 85892-000, Santa Helena, Paraná, Brazil.
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Patricia da costa Zonetti
  • Patricia da costa Zonetti
  • Department of Agronomy, Z Federal University of Paraná, ip code: 85950-000, Palotina, Paraná, B Brazil.
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Ana Paula Ferro
  • Ana Paula Ferro
  • Department of Biochemistry, University of Maringá, Zip code: 87020-900, Maringá, PR, Brazil.
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Osvaldo Ferrarese- Filho
  • Osvaldo Ferrarese- Filho
  • Department of Biochemistry, University of Maringá, Zip code: 87020-900, Maringá, PR, Brazil.
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Maria de Lourdes Lucio Ferrarese
  • Maria de Lourdes Lucio Ferrarese
  • Department of Biochemistry, University of Maringá, Zip code: 87020-900, Maringá, PR, Brazil.
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  •  Received: 31 August 2015
  •  Accepted: 20 November 2015
  •  Published: 14 July 2016


Barros J, Serk H, Granlundz I, Pesquet E (2015). The cell biology of lignification in higher plants. Ann. Bot. doi:10.1093/aob/mcv046.


Boerjan W, Ralph J, Baucher M (2003). Lignin biosynthesis. Ann. Rev. Plant Biol. 54:519-546.


BRASIL (2009). Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Secretaria de Defesa Agropecuária. pp. 398-521.


Bray EA, Bailey-Seres J, Weretilnyk E (2000). In: Biochemistry and Molecular Biology of Plants. Buchanan BB, Gruissem W, Russell LJ (2000), editor. Rockville, Maryland USA: American Society of Plant Physiologist. Responses to abiotic stresses. pp. 1158-1203.


Capeleti I, Bonini EA, Ferrarese MLL, Teixeira ACN, Kryzanowski FC, Ferrarese-Filho O (2005). Lignin content and peroxidase activity in soybean seed coat susceptible and resistant to mechanical damage. Acta Physiol. Plant 27(1):103-108.


Céleres (2014). Relatório biotecnologi. Available at:

View>. accessed on: Feb. 17.


Coghlan A (1999). Splitting headache: Monsanto's modified soya beans are cracking up in the heat. Saint Louis: Monsanto. Available at: 

<View>. Accessed on:10 mar. 2015.


COMPANHIA NACIONAL DE ABASTECIMENTO – CONAB (2013). Estudos deprospecção de mercado: safra 2012/2013. Brasília, 2012a. 148p. Accessed on:

<View>. Accessed on: Oct 10, 2014.


Endt DV, Costa P, Zago MK, Zanettini MHB, Pasquali G (2000). Genes de lignificação. Biotecnologia Ciência & Tecnologia. 3(15):152-159.


Escamilla-Trevino LL, Chen W, Card ML, Shih MC, Cheng CL, Poulton JE (2006) Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides. Phytochemistry 67:1651-1660.


Ferrarese MLL, Zottis A, Ferrarese-Filho O (2002). Protein-free lignin quantification in soybean (Glycine max) roots. Biologia (Bratislava) 57(4):541-543.


Ferreira IC (2003). Lignina de tegumentos de sementes de soja (Glycine max (L.) merr.) e suas relações com a resistência aos danos mecânicos. Maringá: Universidade Estadual de Maringá. Dissertação (Mestrado em Agronomia) P. 43.


Gris CF (2009). Qualidade fisiológica de sementes de soja convencional e RR associada ao conteúdo de lignina. Lavras: Universidade Federal de Lavras.Tese (Doutorado em Agronomia).


Harrison LA, Bailey MR, Naylor M, Ream J, Hammond DL (1996). The expressed protein in synthase in glyphosate-tolerant soybeans, 5-enolpyruvylshiquimate-3-phosphate synthase from Agrobacterium sp. Strain CP4, is rapidly digested and is not toxic to mice upon acute administration. J. Nutr. 128:756-761.


Kuiper HA, Kleter GA, Noteborn HPJM, Kok EJ (2001). Assessment of the food safety issues related to genetically modified foods. Plant J. 27(6):503-528.


Lacerda RS, Gomide CA, Fukushima RS, Schmidt RJ, Herling VR (2003). A new standard for the spectrophotometric method aimed to quantify lignin in forages. Archivos de Zootecnia da Universidade de Cordoba, Cordoba – Espanha. P 214.


Morrison IM (1972). A semi-micro method for the determination of lignin and its use in predicting the digestibility of forage crops. J. Sci. Food Agric. 23:455.


Nodari RO, Destro D (2002). Report on the status of soybean crops of Palmeiras das Missões region, RS, crop 2001/2002, planted with conventional varieties and transgenic cultivars. In View> Accessed on: Nov. 23, 2014.


Padgette SR, Kolacz KH, Delannay XD, La Vallee BJ, Tinius CN, Rhodes WK, Otero YI, Barry GF, Eichholtaz DA, Peschke WM, Nida DL, Taylor NB (1995). Development, identification and characterization of a glyphosate tolerant soybean line. Crop Sci. 35:1451-1460


Shan DM, Horsch RB, Klee HJ (1986). Engineering herbicide tolerance in transgenic plants. Science 233 (4762):478-481.


Terashima N, Yoshida M, Hafre’n J, Fukushima K, Westermark U (2012). Proposed supramolecular structure of lignin in softwood tracheid compound middle lamella regions. Holzforschung 66:907-915.


Vilar FCM, Siqueira-Soares RC, Finger-Teixeira A, Oliveira DM, Ferro AP, Rocha GJ, Ferrarese MLL, Dos Santos WD, Ferrarese-Filho O (2014). The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods. Plos One 9(10):e110000.