African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Study of chlorophyll and macromutations induced by gamma rays and sodium azide in urd bean (Vigna mungo L. Hepper).

Kousar Makeen1, Suresh B. G.1, Lavanya G. R.1 and Archana Kumari2*
1Department of Genetics and Plant Breeding, Allahabad Agricultural Institute–Deemed University, Allahabad – 211007, India. 2Department of Microbiology, Ch. Charan Singh University, Meerut, U.P. India.
Email: [email protected]

  •  Accepted: 24 January 2011
  •  Published: 03 December 2013


The mutagenic effect of gamma rays (5, 10, 20, 30, 40, 50 and 60 kR) and sodium azide (1, 2, 3, 4, 5, 6 and 7 mM) alone or in combination (10 kR + 2 mM, 20 kR + 2 mM, 30 kR + 2 mM and 40 kR + 2 mM) on frequency and spectrum of chlorophyll and micromutations in cultivar, T9 of urd bean have been observed. The spectrum of chlorophyll mutations consisted of albina, chlorina, viresence, viridis and xentha. Out of these chlorophyll mutations, xantha type was predominant in both mutagenic treatments. Conclusively, the combination treatments have yielded the higher frequency, and various doses of mutagenic agents have independent response towards macromutations in bean cv-T9. These studies were broadly aimed at understanding the process of mutation, testing the efficacy of various mutagens, identifying optimum dose and best method of treatment; isolation of mutants of basic and applied value; elucidating the biological effects of gamma rays and sodium azide.


Key words: Gamma rays, sodium azide (SA), chlorophyll, macro mutations, urd bean.


RBD, Randomized block design, SA, sodium azide.