African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Sequence analysis and expression of the calcium-dependent protein kinase gene, MCDPK in mulberry (Morus L.)

Zhang Ying Hua1,2, Lin Qiang1,3, Tong Wei1,2, Liu Zhao Yue1,2, Fang Rong Jun1,2, Zhao Wei Guo1,2* and Li Long1,2
1College of Biological and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China. 2Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu 212018, China. 3The General Station of Sericulture Technology Extension of Guangxi Zhuang Automomous Region, Nanning 530007, China.
Email: [email protected]

  •  Published: 12 June 2012

Abstract

A full-length cDNA sequence coding for calcium-dependent protein kinase of mulberry tree, which we designated was MCDPK and this was cloned based on mulberry expressed sequence tags (ESTs). Sequence analysis showed that the MCDPK is 2249 base pairs in length, encoding 532 amino acids with a predicted molecular weight of 59.96 kDa and an isoelectric point of 6.84. The mRNA expression level ofMCDPK at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCDPK transcripts were most abundantly expressed in the terminal bud, and the expression level of the mRNA was reduced significantly under the conditions of low temperature and salt stress when compared to the normal growth environment. The findings of the study laid the foundation for understanding the resistance mechanism of functional genes in mulberry in the future.

 

Key words: Mulberry, calcium-dependent protein kinase gene (CDPK), gene cloning, abiotic stress, gene expression.