African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

Application of sequence specific amplified polymorphism (SSAP) and simple sequence repeat (SSR) markers for variability and molecular assisted selection (MAS) studies of the Mexican guava

Campos-Rivero G.
  • Campos-Rivero G.
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, C. P. 97200, Mérida, Yucatán, México.
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Cazares-Sanchez E.
  • Cazares-Sanchez E.
  • Instituto Tecnológico de la Zona Maya, carretera Chetumal Escárcega Km. 21.5. C.P. 77960. Ejido Juan Sarabia, Quintana Roo, México.
  • Google Scholar
Tamayo-Ordonez M. C.
  • Tamayo-Ordonez M. C.
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, C. P. 97200, Mérida, Yucatán, México.
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Tamayo-Ordonez Y. J.
  • Tamayo-Ordonez Y. J.
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, C. P. 97200, Mérida, Yucatán, México.
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Padilla-Ramírez J. S.
  • Padilla-Ramírez J. S.
  • Campo Experimental Pabellón, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pesqueras (INIFAP), Pabellón de Arteaga, C. P. 20660, Aguascalientes, México.
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Quiroz-Moreno A.
  • Quiroz-Moreno A.
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, C. P. 97200, Mérida, Yucatán, México.
  • Google Scholar
Sanchez-Teyer L. F.
  • Sanchez-Teyer L. F.
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, C. P. 97200, Mérida, Yucatán, México.
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  •  Received: 01 May 2017
  •  Accepted: 10 July 2017
  •  Published: 20 July 2017

Abstract

Molecular markers have proven to be powerful tools in research related with diversity, variability, and improvement of economically important tropical crops. This study analyzed eight physiological and morphological fruit characters of economic interest in the cultivated Mexican guava (Psidium guajava L.), and assessed the suitability of two sequence specific amplified polymorphism (SSAP) and simple sequence repeat (SSR) markers developed for their use in early selection of individual plants with given fruit characteristics. Principal component analysis (PCA) explained 79% of the morphological variability observed among accessions. S-SAP was more informative than AFLP for studies of variability and diversity in guava, the former marker showing higher percentage of polymorphism (90%) and more intraspecific variability (0.58). It was analyzed by cluster analysis using the unweighted pair group with arithmetic means (UPGMA) method the relationships between accessions from nine guava varieties. S-SAP dendrograms clustered varieties in better agreement with pulp color and fruit shape, suggesting a possible association of the S-SAP marker with quantitative trait loci (QTL) related to fruit physiological and morphological fruit characters. According to results, the microsatellites mPgCIR131, mPgCIR136 and mPgCIR161 might also be linked to QTL related to internal and external pulp thickness, pulp color, and soluble solids, indicating that the SSR markers developed are appropriate for their use in early selection of guava individuals having specific fruit features, therefore being suitable for molecular marker assisted selection (MAS) of the crop.

Key words: Microsatellites, quantitative trait loci (QTL), sequence specific amplified polymorphism (SSAP), simple sequence repeat (SSR), Psidium guajava L.