African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Variations in α-, β-amylase and α-glycosidase activities in two genotypes of wheat under NaCl salinity stress

Chiraz Chaffei Haouari1,2*, Afef Hajjaji Nasraoui1,  Elisa Carrayol2 and Houda Gouia1
1Unité de Recherche: Nutrition et Métabolisme Azotés et Protéines de Stress (99UR/09-20), Département de Biologie, Faculté des Sciences de Tunis. Campus Universitaire El Manar I, 1060, Tunis, Tunisie. 2Unité de Nutrition Azotée des Plantes, INRA, route de Saint Cyr, 78027 Versailles, France.
Email: [email protected]

  •  Accepted: 11 June 2012
  •  Published: 16 May 2013

Abstract

Two wheat differing in salt sensivity, was examined for osmolyte contents and activities of a-amylase, b-amylase and a-glucosidase enzymes involved in seeds germination, in absence as well as in presence of 100, 150, 200 and 300 mM NaCl. The inhibitory effects of NaCl differed, depending on the species tested. In wild wheat specie (Triticum monococcum), with reduced germination percentage and lower relative water content, the increase in NaCl concentration resulted in the decrease in endogenous level of proline, total soluble carbohydrate and activities of the main enzymes involved in the germination process. In contrast, cultivated wheat specie (Triticum aestivum) seed in response to salt stress accumulated higher proline and total soluble carbohydrate concentrations which improved their water status and the enzyme activities involved in the germination process. Differential response of the different species of wheat to salt stress is governed by the accumulation of osmolytes in seeds.

 

Key words: Salinity, amylases, glucosidases, wheat species.