African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6860

Full Length Research Paper

Efficient production of cellulase and xylanase by anaerobic rumen microbial flora grown on wheat straw

Samira Bahramian1*, Mohammad Chamani2, Mehrdad Azin3 and Abbas Gerami4  
1Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. 3Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran. 4The college of Science, Department of Mathematics, University of Tehran, Tehran, Iran.
Email: [email protected]

  •  Accepted: 25 August 2010
  •  Published: 18 June 2011

Abstract

Rumen bacteria, fungi and protozoa possess a wide range of enzymes which are capable of hydrolyzing most of the structural polysaccharides of the plant cell walls. In the study, the activities of cellulolytic and xylanolytic enzymes produced by anaerobic rumen flora were investigated. Mixed ruminal microorganisms were grown on wheat straw in anaerobic liquid medium that have been purged with oxygen-free CO2 and allowed to ferment for 0, 24, 48, 72 and 120 h at 39°C. Then the supernatants of each culture were analyzed for cellulase and xylanase activities with dinitrosalicylic acid (DNS) method. Results showed that optimum time for maximum activity of both enzymes is 48 h incubation at 39°C and at each time pointed, xylanase activity was much higher than cellulase activity.

 

Key words: Rumen microbial flora, cellulase, xylanase.

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