Fusarium oxysporum f.sp. lycopersici isolate NKSC02 showed the highest virulence for wilt incidence of tomato var Sida. Culture of the antagonistic fungus Emericella rugulosa on PDB+CWDB at pH 5 - 8 gave the highest significant fresh weight of fungal biomass which served as an appropriate medium for increasing the number of spores that produces bioformulation. The ED50 of crude ethyl acetate from E. rugulosa against F.oxysporum f.sp. lycopersici isolate NKSC02 was 138 μg/ml, while crude hexane and crude methanol were 313 and 1372 μg/ml, respectively. Tajixanthone, a pure compound of E. rugulosa, expressed antifungal activity against mycelial growth, macrocondia and microconidia of F. oxysporum f. sp. lycopersici NKSC02 with ED50of 122, 54 and 42 μg/ml, respectively. It implies a role of antibiosis. Disease immunity to Fusarium wilt in Sida variety showed the highest immunity of 80.95% when treated with crude EtoAC at 1000 μg/ml, followed by crude EtoAC at 500 μg/ml, with an immunity of 30.09%. Bio-formulations produced from E. rugulosa in powder and oil bases gave highly significant difference in disease severity index (DSI) when compared to the non-treated control. Oil based bioformulation gave higher significant growth parameters and yield than powder based formulation, culture filtrate of E. rugulosa and chemical fungicide treatment; it also lowered wilt incidence of tomato than the others.
Key words: Emericella rugulosa, Fusarium oxysporum f. sp. lycopersici, crude extracts, tajixanthone, bioformulation.
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