African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Increased phenylpropanoid accumulation in essential oils of Petroselinum crispum at different Sulphur dilutions

Giuliana Zardeto
  • Giuliana Zardeto
  • Pharmacy Course, Universidade Paranaense UNIPAR, Praça Mascarenhas de Moraes, 4282, cx. p. 224, CEP 87502-210, Umuarama, Paraná, Brazil.
  • Google Scholar
Denise Akemi Fujisawa
  • Denise Akemi Fujisawa
  • Pharmacy Course, Universidade Paranaense UNIPAR, Praça Mascarenhas de Moraes, 4282, cx. p. 224, CEP 87502-210, Umuarama, Paraná, Brazil.
  • Google Scholar
Keila Fernanda Raimundo
  • Keila Fernanda Raimundo
  • Postgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR, Praça Mascarenhas de Moraes, 4282, cx. p. 224, CEP 87502-210, Umuarama, Paraná, Brazil.
  • Google Scholar
Carla Maria Mariano Fernandez-Andrade
  • Carla Maria Mariano Fernandez-Andrade
  • Postgraduate Program in Pharmaceutical Sciences, Universidade Estadual de Maringá UEM, Av. Colombo, 5.790, Jardim Universitário, CEP 87020-900, Maringá, Paraná, Brazil.
  • Google Scholar
Fabiana Borges Padilha Ferreira
  • Fabiana Borges Padilha Ferreira
  • Postgraduate Program in Health Sciences, Universidade Estadual de Maringá UEM, Av. Colombo, 5.790, Jardim Universitário, CEP 87020-900, Maringá, Paraná, Brazil.
  • Google Scholar
Jose Eduardo Gonçalves
  • Jose Eduardo Gonçalves
  • Postgraduate Programs in Clean Technologies and Health Promotion, Centro Universitário de Maringá, UniCESUMAR, Av. Guedner, 1610, Jardim Aclimação, CEP 87.050-900, Maringá, Paraná, Brazil.
  • Google Scholar
Ranulfo Piau Junior
  • Ranulfo Piau Junior
  • Postgraduate Program in Animal Science, Universidade Paranaense- UNIPAR, Praça Mascarenhas de Moraes, 4282, cx. p. 224, CEP 87502-210, Umuarama, Paraná, Brazil.
  • Google Scholar
Orlando Seiko Takemeura
  • Orlando Seiko Takemeura
  • Pharmacy Course, Universidade Paranaense UNIPAR, Praça Mascarenhas de Moraes, 4282, cx. p. 224, CEP 87502-210, Umuarama, Paraná, Brazil.
  • Google Scholar
Simone de Melo Santana Gomes2
  • Simone de Melo Santana Gomes2
  • Postgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR, Praça Mascarenhas de Moraes, 4282, cx. p. 224, CEP 87502-210, Umuarama, Paraná, Brazil.
  • Google Scholar
Zilda Cristiani Gazim*
  • Zilda Cristiani Gazim*
  • Pharmacy Course, Universidade Paranaense UNIPAR, Praça Mascarenhas de Moraes, 4282, cx. p. 224, CEP 87502-210, Umuarama, Paraná, Brazil.
  • Google Scholar


  •  Received: 17 June 2016
  •  Accepted: 17 August 2016
  •  Published: 22 September 2016

Abstract

This study aimed to evaluate the influence of different doses of Sulphur on yield and chemical composition of parsley essential oil. Sulphur was applied at different dilutions in the centesimal scale ranging from 0c (control), 6, 12, 18, 24 to 30c. The experiment was carried out in the field, and for each treatment there were five replications, that is, five vases with one plant each. The evaluated parameters were: plant height (cm), fresh biomass of the aerial parts and root (g), yield and chemical composition of the essential oil. The oil was obtained by hydrodistillation and analyzed by gas chromatography and mass spectrometry (GC/MS). The results indicated that 12c dilution caused aerial part inhibition; there was an inhibitory effect on roots at 12 and 30c dilutions, and 6, 12 and 30 c dilutions inhibited fresh biomass when compared to the control (0c) and the other dilutions. Regarding EO yield, an increase in yield (%) at 18c (0.150 ± 0.01) and 30c (0.180 ± 0.01) dilutions occurred when compared to control (0c) (0.017 ± 0.01). The essential oil presented phenylpropanoids as the main class in its composition and apiole and myristicin as major compounds in all evaluated treatments. The 12c dilution allowed an increase in apiole (96.24%) and decrease in myristicin (3.76%).  However, myristicin has an increase in treatments 6c (14.65%), 18c (10.46%), 24c (13.66%) and 30c (14.80 when compared to the control 0c (5.99%).  In conclusion, a stimulating or inhibitory response occurred in the evaluated parameters depending on the utilized dilutions. The increase in apiole and myristicin is considered an economically important factor because they are substances utilized by the pharmaceutical, food and agricultural industries. 

Key words: Parsley, apiole, myristicin, phenylpropanoids, yield, dilutions.