The variability in Agro-morphological and morphometric of 30 pigeon pea genotypes in two environments using seed image analysis was investigated. The experimental seeds were sourced from IITA, and IAR&T, Ibadan, Nigeria. An EPSON scanner was connected to a computer device to acquire an image of the seeds and the Regent instrument was used for the image analysis. For every replication, hundred seeds were placed on the lighting hood and analysed by the scanner and the image of the seed was recorded by the WinSEEDLETM. The procedure was repeated three times for all the genotypes and data collected were subjected to ANOVA, mean separation was done using Tukey HSD at 5% level of probability. The result showed that, the seed production environment effect and genotype effect were highly significant (p<0.01) on all the seed morphometric parameters examined. Highest value of seed area and seed straight length was recorded in genotype NSWCC-34 (38.76mm2) and NSWCC-34b (7.45mm). Seed morphometric attributes were variable among some genotypes implying that, it should be given due consideration in seed conditioning and improvement. Genotypes NSWCC-34C, NSWCC-34b, NSWCC-50 and NSWCC-7D were identified with consistent and high seed morphometric characteristic performance for most of the attributes evaluated within and across the two seed production environments hence recommended.

Keywords: seed scanner, seed simprovement, seed conditioning.