Heavy metals are one of great importance and have a notable adverse effect leading to hazardous crop growth. Plants have a susceptibility to heavy metal toxicity and respond to avoid detrimental effects in a variety of crops. Our study is revealed that the metal toxicity after elevating on particular concentration and different stages of development. The seeds of Brassica nigra were used to evaluate the effect of iron (Fe) on different physiological parameters at different stage of development. The effect of Fe was studied with regard to seed germination, root length, leaf area and total chlorophyll under 50 µM, 100 µM and 200 µM of concentrations. These concentrations significantly affect the seed germination, root length, leaf area and total chlorophyll. Treatment was given from seed stage to the mustard seeds for evaluation of germination percentage in petridish, Fe exhibited a reduction in seed germination and root length but when treatment was given into pot after three leaves emerging stage, Fe was not exhibited toxic effect upto at 200 µM concentration.

Keywords: Brassica nigra, Heavy Metals, Iron