The study was conducted in the selected districts of East Wollega Zone, Oromia Regional State, Ethiopia, to determine the prevalence of honeybee disease and pests. A questionnaire survey and laboratory diagnostic methods were used for the study. The questionnaire was administered to 146 beekeepers (97.1% males). Two honeybee colony samples from each beekeeper, totally (292 honeybee colonies), were collected from transitional and frame box hives for laboratory diagnosis. The honeybee samples collected were examined in the laboratory for the prevalence of honeybee disease pathogens and pests. The prevalence of Varroa and bee lice as honeybee pests and Nosema, Amoeba and Calk brood disease as pathogen was confirmed. However, tracheal mite, stone brood, American and European foul brood were not confirmed during the study period. The prevalence of varroa destructor was higher in a dry active season, while the prevalence of Nosema and Chalk brood disease was limited during the dry season. Amoeba disease was occurred in both seasons.

Keywords: Disease, Honeybees, Infestation, Oromia, Pests, Prevalence