African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

In silico characterization and evolution studies of alcohol dehydrogenase gene from Phoenix dactylifera L.cv Deglet Nour

Imen Rekik*
  • Imen Rekik*
  • Laboratory of plant biotechnology, Faculty of sciences of Sfax, University of Sfax, Tunisia.
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Amine Elleuch
  • Amine Elleuch
  • Laboratory of plant biotechnology, Faculty of sciences of Sfax, University of Sfax, Tunisia.
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Noureddine Drira
  • Noureddine Drira
  • Laboratory of plant biotechnology, Faculty of sciences of Sfax, University of Sfax, Tunisia.
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Foued Cheour
  • Foued Cheour
  • High Institute of Applied Biology of Medenine, Tunisia.
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  •  Received: 21 March 2015
  •  Accepted: 15 May 2015
  •  Published: 20 May 2015

Abstract

The aim of our study was to isolate the alcohol dehydrogenase (ADH) mRNA from Phoenix dactifera, and examine the molecular evolutionary history of this nuclear gene with others ADH genes from palms and other plants species. The DnADH gene has been isolated in silico by BLAST2GO from a cDNA library of date palm cv Deglet Nour. The prediction of candidate’s mRNA and protein for ADH gene from khalas were performed in silico from whole genome shotgun sequence (ACYX02009373.1) using FGENESH prediction program. Nucleotide polymorphism using DnaSPv5 was examined in four palm ADH mRNA sequences across the entire 1.098 kb length of ADH mRNA. A primary conclusion of the present study is that nucleotide diversity for ADH between palm species is very low. In order to assess selective pressure, we calculated the ratio of non-synonymous to synonymous substitutions. We conclude that ADH palms genes appear to be under very different selective constraints. Phylogenetic analyses using PHYLIP and Notung 2.8 programs suggest that ADH genes of some plants species resulted from relatively ancient duplication events. In this study, we present for the first time a molecular characterization of ADH protein of P. dactylifera L cv Deglet nour and a phylogeny analysis between plants ADH.

 

Keys word: Alcohol dehydrogenase, palms species, evolution, duplication.

Abbreviation

EST, Expressed sequence tags; ADH, alcohol dehydrogenase; CDD, conserved domains database; ML, maximum-likelihood; SNP, single nucleotide polymorphisms.