African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Table of Content: 20 May, 2015; 14(20)

May 2015

In silico characterization and evolution studies of alcohol dehydrogenase gene from Phoenix dactylifera L.cv Deglet Nour

The aim of our study was to isolate the alcohol dehydrogenase (ADH) mRNA from Phoenix dactifera, and examine the molecular evolutionary history of this nuclear gene with others ADH genes from palms and other plants species. The DnADH gene has been isolated in silico by BLAST2GO from a cDNA library of date palm cv Deglet Nour. The prediction of candidate’s mRNA and protein for ADH gene from khalas were performed in...

Author(s): Imen Rekik, Amine Elleuch, Noureddine Drira and Foued Cheour

May 2015

Responses of potatoes plants inoculated with arbuscular mycorrhizal fungi and litter in greenhouse

A pot experiment was set to examine the impact of the foliar litter (Hardwickia binata and Azadirachta indica) and an arbuscular mycorrhizal (AM) fungus on the development of two varieties of potato plants (Aida, Atlas). Three litter doses (0, 25 and 50 g) were applied to the pots after bedding plantlets. The plants were inoculated with AM, Glomus aggregatum. Mycorrhizal colonization, shoot dry weight, size and number...

Author(s): Bathie Sarr, Fatimata Ndiaye, Malick Ndiaye, Mame Arame Fall Ndiaye and Tahir Abdoulaye Diop

May 2015

Induced polygenic variability using combination treatment of gamma rays and ethyl methane sulphonate in blackgram (Vigna mungo (L.) Hepper)

Induced mutation in plant improvement has been proven to be one of the alternative ways to generate new sources of genetic variation in blackgram. In this study, dry seeds of VBN 4 blackgram were treated with combination treatment of both gamma rays (400, 500 and 600 Gy) and ethyl methane sulphonate (EMS) (50, 60 and 70 mM) to study the polygenic characters in M2 generation. The mean values for plant height, number of...

Author(s): K. S. Usharani and C. R. Ananda Kumar

May 2015

Growth of Chlorella vulgaris and Nannochloris oculata in effluents of Tilapia farming for the production of fatty acids with potential in biofuels

The use of microalgae in wastewater treatment and its biotechnological exploitation for the production of biofuels is a potential environmental application. Some species of microalgae are notable due to their lipid composition and fatty acid profile suitable for biofuel production. During the present study, a factorial 23 experimental design was conducted, which assessed three factors: i) two species of microalgae...

Author(s): Yesica I. Ferrer-Álvarez, Luis A. Ortega-Clemente, Ignacio A. Pérez-Legaspi, Martha P. Hernández-Vergara, Paula N. Robledo-Narváez, Elvira Ríos-Leal and Héctor M. Poggi-Varaldo

May 2015

Microbiological evaluation and shelf life of seed flour mixes used for infant feeding in rural northern Nigeria

This study investigates microbial load and shelf life of locally processed cereal-legume-oil seed flour mixes used for infant feeding in rural northern Nigeria.  Free fatty acid (FFA), water activity (aw), pH, total viable count (TVC) and presence of yeast, coliform bacteria and mold were the parameters determined. Hungry rice or Digitaria exilis, benne seed or Sesamum indicum and soybean or Glycine max seeds...

Author(s): Ibeanu, V. N., Ene-Obong, H. N., Peter-Ogba, G. U. and Onyechi, U. A.

May 2015

Design of experiment approach for the process optimization of ultrasonic-assisted extraction of polysaccharide from mulberry leaves by response surface methodology

Mulberry is considered as food-medicine herb, with specific nutritional and medicinal values. In this study, response surface methodology (RSM) was employed to optimize the ultrasonic-assisted extraction of total polysaccharide from mulberry using Box-Behnken design (BBD). Based on single factor experiments, a three level, three variable central composite designs were carried out to establish a quadratic regression...

Author(s): Zhao Mengdi#, Zhou Hong#, Wang Heng , Weiguo Zhao, , Hyong-Jun Kim and Liu Li

May 2015

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

Methods for separation of chlorogenic acid (CA) from tobacco by-products were established. The liquid chromatography tandem mass spectrometry (LC-MS) method for identification and analysis of chlorogenic acid from tobacco materials has been developed. CA was isolated by polyamide and further purified by silicagel column chromatography. Results reveal that polyamide is suitable for separation of CA from tobacco extract....

Author(s): Wenwen Zhao, Yuru Chen , Shaofeng Li, Kewei Dai, Yan Chen and Shijie Yang

May 2015

Physical-chemical characteristics and antioxidant potential of seed and pulp of Ximenia americana L. from the semiarid region of Brazil

Ximenia americana popularly known as wild plum grow wildly in Brazilian semiarid region and its fruit were harvested in two maturity stages and evaluated for quality. The experimental design was completely randomized with three treatments (immature, mature pulp and seeds), and treatment effect was evaluated for pulp fraction (composed of both mesocarp and exocarp or peel) in maturation stages:...

Author(s): José Darcio Abrantes Sarmento, Patrícia Lígia Dantas de Morais, Francisco Israel de Souza and Maria Raquel Alcântara de Miranda

May 2015

Acute toxicity and behavioral changes of Caspian kutum (Rutilus frisii Kutum Kamensky, 1991) and Caspian roach (Rutilus rutilus caspicus Jakowlew, 1870) exposed to the fungicide hinosan

Pesticides are used in agriculture to control pest and protect human health and animals. Excessive use of pesticides caused risk for human health and threated non-target organisms, polluted water, soil and air. Hinosan is a component of organophosphate pesticide which is used as a fungicide in agricultural fields. In the study, lethal concentration (LC50) of Hinosan was calculated for Rutilus frisii kutum and Rutilus...

Author(s): Saeid Shahbazi Naserabad, Alireza Mirvaghefi, Mohammad Hasan Gerami and Hamed Ghafari Farsani

May 2015

Cellulolytic activity of gut extract of subterranean termite, Odontotermes obesus Rambur: A pretreatment tool for conversion of lignocellulosic biomass to fermentable sugar for biorefinery industry

Lignocellulosic biomass is a chief and cheap raw material for bioethanol production. However, pretreatment is a critical and most expensive step in lignocellulosic biomass to biofuel conversion. Biological pretreatments offer an alternative for lignocellulosic biomass conversion using enzyme hydrolysis. Termites are well known for the ability to digest lignocelluloses, using it as a sole food source. To effectively...

Author(s): Natchiappan Senthilkumar, Sourimuthu Murugesan and Devaraj Suresh babu

May 2015

Physicochemical and microbiological meat quality, growth performance and carcass characteristics of feedlot-finished cull Santa Inês ewes and Moxotó goats¹

This study aimed at assessing the physicochemical and microbiological quality of feedlot-finished, culled Santa Inês ewes and Moxotó goats, and compares the intake, weight gain and carcass characteristics. Three Santa Inês ewes and Moxotó goats with similar ages were confined in double and/or individual pens and then euthanized after 40 days of confinement. The average intake, daily weight...

Author(s): Beatriz Severino da Silva, Geovana Rocha Plácido, Elis Aparecido Bento, Wellingthon da Silva Guimarães Júnnyor and Marco Antônio Pereira da Silva