African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12489

Full Length Research Paper

Biochemical composition of three Tunisian silverside (fish) populations caught in open sea, lagoon and island coasts

Nawzet Bouriga1, 3, Salah Selmi2*, Eric Faure3 and Monia Trabelsi1
  1Unité de Biologie marine. Faculté des Sciences de Tunis. El Manar II, 2092 Campus universitaire. Tunisie. 2 Institut National des Sciences et Technologies de la Mer INSTM, La Goulette 2060, Tunisie. 3LATP, CNRS-UMR 6632, Evolution biologique et modélisation, case 5, Université de Provence, Place Victor Hugo, 13331 Marseille cedex 3, France.
Email: [email protected]

  •  Accepted: 27 May 2010
  •  Published: 28 June 2010

Abstract

 

Fatty acid and amino acid profiles were determined in three silverside populations caught in Tunisian waters Atherina boyeri (open sea), Atherina lagunae (lagoon) and Atherina sp. (island coasts). Saturated fatty acids reached in total lipids 43.54%, 36.96% in marine and 33.64% in insular silverside and A. lagunae, in which eicosapentaenoic acid, docosahexaenoic acid and linoleic acid were the prominent fatty acids. The n-3/n-6 index showed a significant level indicating a tendency to accumulate n-3 fatty acids in A. boyeri and A. lagunae and n-6 fatty acids inAtherina sp. Total amino acid content ranged from 528 to 588 mg/g crude protein, in which, glutamic acid was the most abundant. Methionine had the lowest essential amino acid score in A. boyeri and Atherina sp. (0.73 and 0.71, respectively) while tryptophan had the lowest in A. lagunae (0.07).

 

Key words: Silverside populations, fatty acids, amino acids, biochemical composition.

Abbreviation

PUFA, Polyunsaturated fatty acids; EPA, eicosapentaenoic acid;DPA, docosapentaenoic acid; DHA, docosahexaenoic acid; FAMEs, fatty acids methyl esters; HPLC,  high performance liquid chromatography; SFA, saturated fatty acids; MUFA, monounsaturated fatty acid; EAA, essential amino acid; P-PER, predicted protein efficiency ratio.