African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

Full Length Research Paper

Assessment of the genetic diversity of African yam bean (Sphenostylis stenocarpa Hochst ex. A Rich. Harms) accessions using amplified fragment length polymorphism (AFLP) markers

Omena B. Ojuederie*
  • Omena B. Ojuederie*
  • Department of Biotechnology, Bells University of Technology, Ota, Nigeria; Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan Nigeria.
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Morufat O. Balogun
  • Morufat O. Balogun
  • Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan Nigeria.
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Iyiola Fawole
  • Iyiola Fawole
  • Department of Biological Sciences, Bells University of Technology, Ota, Nigeria.
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David O. Igwe
  • David O. Igwe
  • Molecular Virology and Diagnostic Laboratory, International Institute of Tropical Agriculture, Ibadan Nigeria.
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Mercy O. Olowolafe
  • Mercy O. Olowolafe
  • Department of Biotechnology, Institute of Agricultural Research and Training, Moor Plantation, Ibadan, Nigeria.
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  •  Received: 17 February 2014
  •  Accepted: 19 April 2014
  •  Published: 30 April 2014

Abstract

The genetic diversity of 40 African yam bean (AYB) accessions was assessed using amplified fragment length polymorphism (AFLP) markers. Seeds of 40 accessions of AYB obtained from the International Institute of Tropical Agriculture (IITA) and Institute of Agricultural Research and Training (IAR&T) Ibadan, Nigeria, were grown in a greenhouse and young leaves from two weeks old plants collected for DNA extraction. The four primer combinations used generated a total of 1730 amplification fragments across the AYB accessions used in this study of which 1647 were polymorphic (95.20%). The number of amplified polymorphic AFLP bands per primer pair varied from 360 to 520 with an average percentage polymorphism of 95.6%. E-AGC/M-CAG produced the highest number of polymorphic bands (520). Polymorphic information content (PIC) values ranged from 0.9447 to 0.9626. The highest level of polymorphism (100%) was recorded for two primer combinations (E-AAC/M-CAG and E-ACT/M-CAG). The results of cluster analysis using UPGMA tree, grouped the 40 accessions of AYB into two major clusters with an overall similarity of 67.5%. The level of similarity between the accessions spanned 0.66 to 0.91. TSs 138 and TSs 139 were the most closely related accessions with high level of similarity index (0.91). Comparable results were obtained using Factorial Coordinate Analysis (FCO).  The results from the present study confirm the robustness and the suitability of the AFLP as a molecular tool for the assessment of genetic diversity in AYB accessions.

 

Key words: Amplified fragment length polymorphism (AFLP), cluster analysis, genetic diversity, Sphenostylis stenocarpa, polymorphism.

Abbreviation

Abbreviation: AFLP, Amplified fragment length polymorphism; AYB, African yam bean; FCO, factorial coordinate analysis; NUS, neglected underutilized species; PIC, polymorphic information content; TSs, tropical sphenostylis stenocarpa; UPGMA, unweighted paired group method using arithmetic average.