African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Table of Content: 30 April, 2014; 13(18)

April 2014

Microbial agents against Helicoverpa armigera: Where are we and where do we need to go?

Plants are prone to various biotic stresses in nature by bacteria, viruses, fungi, parasites, harmful insects and weeds. The biggest percentage loss (70%) in plants is attributed to insects. Lepidoptera is one such diversified phytophagous insect group, which include Helicoverpa armigera, a key pest of many food crops including chickpea, pigeonpea, pea, lentil, chillies, sunflower, tomato, tobacco and cotton....

Author(s): Rajendran Vijayabharathi, Bhimineni Ratna Kumari and Subramaniam Gopalakrishnan

April 2014

Cloning of partial cry1Ac gene from an indigenous isolate of Bacillus thuringiensis

The discoveries of novel cry genes of Bacillus thuringiensis (Bt) with higher toxicity are important for the development of new products. The cry1 family genes are more toxic to the lepidopteran insects ­­­­­­according to the previous reports. In the present study, nine indigenous isolates of Bt were used for screening of cry1 genes by PCR using degenerate primers specific to cry1 family genes....

Author(s): R. Manikandan, A. Ramalakshmi, V. Balasubramani and V. Udayasuriyan

April 2014

Assessment of the genetic diversity of African yam bean (Sphenostylis stenocarpa Hochst ex. A Rich. Harms) accessions using amplified fragment length polymorphism (AFLP) markers

The genetic diversity of 40 African yam bean (AYB) accessions was assessed using amplified fragment length polymorphism (AFLP) markers. Seeds of 40 accessions of AYB obtained from the International Institute of Tropical Agriculture (IITA) and Institute of Agricultural Research and Training (IAR&T) Ibadan, Nigeria, were grown in a greenhouse and young leaves from two weeks old plants collected for DNA extraction. The...

Author(s): Omena B. Ojuederie, Morufat O. Balogun, Iyiola Fawole, David O. Igwe and Mercy O. Olowolafe

April 2014

Microbial composition of guava (Psidium guajava), hibiscus (Hibiscus-rosa sinensis), mango (Mangifera indica) and pumpkin (Telfairia occidentalis Hook) phyllosphere

Diverse groups of microorganisms colonize phyllosphere and carry out different but specific ecological functions. The phyllosphere of four different plants; guava (Psidium guajava), hibiscus (Hibiscus-rosa sinensis), mango (Mangifera indica) and pumpkin (Telfairia occidentalis Hook), each from four different sites Aluu Community Health Centre, Lulu Briggs Health Centre, University of Port Harcourt Abuja campus, and...

Author(s): C.B. Chikere and C.C. Azubuike

April 2014

Genetic evaluation of spring wheat (Triticum aestivum L.) genotypes for yield and spot blotch resistance in Eastern Gangetic Plains of India

An experiment was conducted to evaluate 49 spring wheat (Triticum aestivum L.)  genotypes of diverse origin by estimating genetic parameters viz. variability, character association, path coefficient, cluster and principle component analysis (PCA) for yield and spot blotch disease resistance during 2011 -2012 and 2012 - 2013. Highest phenotypic coefficient of variation (PCV) was observed for area under disease...

Author(s): Nitish De, D. K. Baranwal and Sudhir Kumar

April 2014

Certain growth related attributes of bunchy top virus infected banana under ex-vitro conditions

Effect of banana bunchy top virus (BBTV) on morpho-physiological characteristics of banana (Musa sp.) cv., Basrai plants was assessed. Healthy and BBTV infected samples of banana were collected from its open fields and micro-propagated aseptically. These plantlets were established in wire-house for three months. Presence of BBTV was confirmed by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay...

Author(s): Ikram-ul-Haq, Shahrukh, Um-E-Aiman, Saifullah Khan, Nazia Parveen, Madiha Fatima and Muhammad Umar Dahot

April 2014

Comparative evaluation of organic and conventional farming on chemical quality parameters and antioxidant activity in fruits

The aim of this research was to determine some important quality parameters and the antioxidant activity of organic and conventional  oranges, limes and apples. Five samples of organic fruits with seal certification, organic fruits without seal certification and conventional fruits were acquired from supermarkets and farm in Rio de Janeiro, Brazil. Organic lime and orange showed higher mean values of acidity, being...

Author(s): Danielle dos Santos Bonfim de Castro, Lana de Souza Rosa, Ellen Mayra da Silva Menezes and Anderson Junger Teodoro

April 2014

In vitro antimicrobial potential of organic solvent extracts of novel actinomycetes isolated from forest soil

In vitro screening of antibacterial and antifungal activities of hexane, chloroform, ethyl acetate, methanol and water extracts of selected promising actinomycetes strains were studied towards Gram-positive, Gram-negative bacteria, dermatophytes and opportunistic pathogens. Crude antimicrobial metabolites were extracted by liquid-liquid extraction and solid-liquid extraction method using hexane, chloroform, ethyl...

Author(s): M. Valan Arasu, T.S.Rejiniemon, N. A. Al-Dhabi, V. Duraipandiyan, P. Agastian,  V.A.J. Huxley, C.E. Song and K.C Choi

April 2014

cDNA cloning, characterization and expression of cytochrome P450 family 1 (CYP1A) from Javanese medaka, Oryzias javanicus by environmental conditions

Cytochrome P450 family 1 (CYP1A) subfamily genes are the most well studied CYP genes in vertebrates; however, information on CYP1A genes in Javanese medaka is relatively scarce. In the present study, full-length cDNA of CYP1A was cloned from the fish liver exposed to 500 ppb β-naphthoflavone for 24 h, which is 2439 bp contained in an open reading frame of 1593 bp encoding a protein of 530 amino acids. Real time...

Author(s): Trieu Tuan, Yoshino Kaminishi, Aki Funahashi, El-Kady A.H. Mohamed, Abeer A. I. Hassanin and Takao Itakura

April 2014

Evaluation of essential trace metals in female type 2 diabetes mellitus patients in Nigerian population

Diabetes mellitus (DM)  has been reported to be associated with derangement of micronutrients. This study was to investigate the plasma levels of antioxidant elements (zinc, selenium, copper) haemopoeitic elements (chromium and iron) and magnesium in female type 2 diabetes mellitus (DM) patients. Fifty (50) female type 2 DM patients (test subjects) aged 20 to 50 years and 35 healthy, non-diabetic age-matched...

Author(s): Samuel Oyewole Oyedeji, Adeyemi Adeleke Adesina, Olusegun Taiwo Oke and Yetunde Olufunmilayo Tijani

April 2014

Investigation of carrier oil stabilized iron oxide nanoparticles and its antibacterial activity

Iron oxide nanoparticles were synthesized by co-precipitation method. The polyunsaturated carrier oil (flaxseed oil) is used as a stabilizing agent for iron oxide nanoparticles. Kirby Bauer method was used to investigate the antibiotic sensitivity of carrier oil stabilized and uncoated SPIONs at 10 and 20 μg/L on Gram-positive bacterium Bacillus cereus (vegetative cell). The nanoparticles were characterized by X-ray...

Author(s): K. L. Palanisamy, V. Devabharathi and N. Meenakshi Sundaram