Abstract

Maillard reaction (MR) was carried out in L-lysine-D-glucose (Lys-Glu) model system heated at 120°C for 0 to 10 h without pH control. Optical property (UV-Vis absorbance and fluorescence) development of MR was monitored. Antioxidant activity of maillard reaction products (MRPs) was investigated by a series of in vitrotests. The UV-Vis absorbance zero and first-order derivative spectrophotometric was utilized to study the metal-binding property and chelating effect of different fractions of MRPs. MRPs had reducing power, strong free radical-scavenging activity and copper (II)-binding ability. The antioxidant activity of MRPs related well with the UV-Vis absorbance properties of maillard rection. Cu²⁺-binding to MRPs caused the electronic rearrangement in UV-Vis absorbance intermediates, but had no effect on the chromophore structure in brown polymers. The UV-Vis absorbance compounds were responsible to the reducing power and radical-scavenging activity. The fractions of low molecular weight MRPs showed effective metal-chelating ability.

Key words: Maillard reaction products, antioxidant activity, metal-chelating effect, optical properties.

Abbreviation

MR, Maillard reaction; MRPs, maillard reaction products; TMM,tetramethylmurexide; DPPH, 2,2-diphenyl-1-picrylhydrazyl.