July 2011
Suitability of a selected set of simple sequence repeats (SSR) markers for multiplexing and rapid molecular characterization of African rice (Oryza glaberrima Steud.)
African rice (Oryza glaberrima) was neglected for a long time by researchers but gained attention with the success of NERICA varieties. While Africa rice holds a collection of about 2,500 O. glaberrima samples only four accessions have been used as NERICA parents. This collection needs to be characterized using rice descriptors and molecular markers to fully exploit the whole diversity of this...
July 2011
Assessment of genetic diversity in different clones of Dalbergia sissoo Roxb. by RAPD markers
Genetic diversity of forty (40) clones of Dalbergia sissoo Roxb was analyzed using randomly amplified polymorphic DNA (RAPD) markers by selecting 30 decamer primers, which were later reduced to 10 based on the preliminary PCR amplification. A total of 129 distinct DNA fragments (bands) were amplified, of which 104 bands were polymorphic ranging from 200 to 1300 base...
July 2011
Special variations within 11.7 kb fragment in goat polled intersex syndrome
The goat polled intersex syndrome (PIS) is a full association between the absence of horns and intersexuality. Several pairs of primer were designed to detect the complete or partial deletion of 11.7 kb fragment in PIS goat. We proved that the 11.7 kb fragment was not deleted completely but partially in PIS goat. The special 198 base substitutions were found between normal...
July 2011
Expression optimisation of recombinant α-L-arabinofuranosidase from Aspergillus niger ATCC 120120 in Pichia pastoris and its biochemical characterisation
A gene encoding α-L-arabinofuranosidase (AnabfA) from Aspergillus niger ATCC 120120 was successfully cloned and expressed in Pichia pastoris under the control of the AOX1 promoter. The effect of cultural conditions on recombinant AnabfAproduction was studied and the enzyme was expressed as a soluble protein. Recombinant AnabfA was expressed as an active enzyme at...
July 2011
Studies of the genetic diversity of seven sweetsop (Annona squamosa L.) cultivars by amplified fragment length polymorphism analysis
Seven sweetsop germplasm resources were studied, and amplified fragment length polymorphism (AFLP) analysis system was established by detecting the results of several reactions, such as DNA extraction, enzyme restriction, ligation, preamplification and selective amplification in the reaction system of AFLP. The seven germplasm resources of sweetsop were studied by using AFLP technique, and UPGMA clustering...
July 2011
Interspecific hybridization between Mauremys reevesii and Mauremys sinensis: Evidence from morphology and DNA sequence data
Turtle hybrids have been found in some taxa, but so far, studies on interspecific hybridization between Mauremy reevesii and Mauremy sinensis have not been reported. Recently, we obtained three specimens (Hy1, Hy2, Hy3）with unusual morphological characteristics from pet trade Market, which are suspected to be hybrids rather than new species, because they were morphologies...
July 2011
Cloning and mRNA expression pattern analysis under low temperature stress of EAAP gene in Dongmu-70 rye
This research cloned endochitinase-antifreeze protein precursor (EAPP) gene of Dong-mu 70 rye (Secale cereale) by designing special primers according to Genbank’s EAPP gene sequence, and analyzing the influence of low temperature stress on the expression of mRNA with RT-PCR. The results indicated that theEAPP’s full length coding of Dong-mu-70 rye was 1085 bp, while the homology of...
July 2011
Effects of soil mesofauna and microclimate on nitrogen dynamics in leaf litter decomposition along an elevation gradient
The nutrient dynamics in decomposing litter has proved to play a crucial role in regulating the nutrient status of ecosystems and vegetation productivity. Little is known, however, about the effect mechanism of decomposer and its environment on the nitrogen (N) mineralisation of litter along an elevation gradient in such sites. We investigated the effects of soil mesofauna using litter bags on N dynamics in...
July 2011
Effect of soaking of seeds in potassium silicate and uniconazole on germination and seedling growth of tomato cultivars, Seogeon and Seokwang
Experiments were conducted to investigate the effects of soaking seeds in potassium silicate and uniconazole on seed germination and seedling growth of two tomato cultivars. Tomato (Lycopersicum esculentum Mill. ‘Seogeon and Seokwang’) seeds were put in a Petri dish filled with 15 ml of a solutions containing either 50 or 100 mg L-1 potassium silicate or uniconazole and were placed...
July 2011
Investigation of branch breaking resistances in “Sari Zeybek†fig cultivar
The aim of this study was to examine the branch breaking characteristics of the 4 to 5 years old branches mechanically by a determination of the breaking resistance in Sari Zeybek fig cultivars. In this respect, the study was conducted with 10 different ages for trees belonging to Sarilop cultivar, known with a characteristic of drying and Sari Zeybek cultivar, known with equal or even better...
July 2011
Efficient plant regeneration from cotyledonary node explants of Cucumis melo L.
An efficient regeneration system was developed using cotyledonary node of two inbred line of melon, Cucumis melo 'CM-15' and 'CM-23'. The induction of shoots was achieved on Murashige and Skoog (MS) solid medium supplemented with different combinations of 6-benzylaminopurine (BA) and indole-3-acetic acid (IAA). The best shoot induction was observed when the explants were...
July 2011
Al-induced root cell wall chemical components differences of wheat (Triticum aestivum L.) differing in Al tolerance
Root growth is different in plants with different levels of Al-tolerance under Al stress. Cell wall chemical components of root tip cell are related to root growth. The aim of this study was to explore the relationship between root growth difference and cell wall chemical components. For this purpose, the cell wall chemical components of root tip cell in two near-isogenic lines (NIL) wheat (Triticum...
July 2011
In vitro investigation of the hypoglycemic activity of yeasts using models of rat epididymal adipocyte and differentiated mouse 3T3-L1 adipocyte
The differentiated mouse 3T3-L1 adipocytes (3T3-L1 model) were used in studying glucose metabolisms without the need for feeding (Sprague-Dawley, SD model) the rat prior to hypoglycemic activity evaluation. Both models were adopted to evaluate the hypoglycemic activities of 58 yeast strains isolated from various sources (grape, vine yard soil, winery soil). Among the 58 tested yeast isolates, strain...
July 2011
Antioxidant activity of maillard reaction products from lysine-glucose model system as related to optical property and copper (II) binding ability
Maillard reaction (MR) was carried out in L-lysine-D-glucose (Lys-Glu) model system heated at 120°C for 0 to 10 h without pH control. Optical property (UV-Vis absorbance and fluorescence) development of MR was monitored. Antioxidant activity of maillard reaction products (MRPs) was investigated by a series of in vitrotests. The UV-Vis absorbance zero and first-order derivative spectrophotometric was...
July 2011
Inhibitory mechanism of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol synthesis with Klebsiella pneumoniae
3-Hydroxypropionaldehyde accumulation may cause the cessation of 1,3-propanediol sustained production with glycerol by Klebsiella pneumoniae. The impeller tip speed shift from higher to lower speed at glycerol excess or the pulsed glycerol feeding could lead to an abrupt increase of the 3-hydroxypropionaldehydeconcentration (up to 10 mmol/l) in 10...
July 2011
Antimicrobial efficacy of nanosilver, sodium hypochlorite and chlorhexidine gluconate against Enterococcus faecalis
The purpose of this study was to compare the antibacterial efficacy of nanosilver (NS), chlorhexidine gluconate (CHX) and sodium hypochlorite (NaOCl) againstEnterococcus faecalis. Two tests of minimum inhibitory concentration (MIC) and zone of inhibition were carried out using NS, NaOCl and CHX. 70-fold concentration of NaOCl is required for the same antibacterial effect of NS. CHX precipitated in...
July 2011
Effect of garlic (Allium sativum) on Salmonella typhi infection, gastrointestinal flora and hematological parameters of albino rats
The effect of consumption of garlic (Allium sativum) in treating Salmonella typhiinfection and on the gastrointestinal flora and hematological parameters of rats was investigated. Crude garlic extract inhibited the growth of S. typhi on agar plate with a zone of inhibition averaging 23.8 mm in diameter using the agar diffusion assay. This inhibition was superior to that of all the conventional...
July 2011
Polo-like kinase 1 siRNA-607 induces mitotic arrest and apoptosis in human nasopharyngeal carcinoma cells
Polo-like kinase (Plk) 1 is overexpressed in many human malignancies including nasopharyngeal carcinoma, indicating its potential as a therapeutic target. Recently, using a simple cellular morphology-based strategy, we have identified several novel effective siRNAs against Plk1 including Plk1 siRNA-607. In this study, we further investigated the effects of Plk1 siRNA-607 in human nasopharyngeal carcinoma...
July 2011
Expression of human soluble tumor necrosis factor (TNF)-related apoptosis-inducing ligand in transplastomic tobacco
The soluble extracellular domain of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (sTRAIL) can, as the whole length TRAIL protein, bind with its receptors and specifically induce the apoptosis of cancer cells; therefore, it has been developed as a potential therapeutic agent for various cancer treatments. As it has become an attractive technology for foreign protein production,...
July 2011
Improvement of acid protease production by a mixed culture of Aspergillus niger and Aspergillus oryzae using solid-state fermentation technique
The synthesis of acid protease by Aspergillus oryzae AS3042 was enhanced significantly with the mixed culture of Aspergillus niger SL-09 using solid-state fermentation technique. The influence of carbon sources, nitrogen sources and the addition of phytic acid on acid protease production were investigated. The enzyme production varied depending on the nitrogen source. Apart from that, it...
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