African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Antioxidant activity of some African medicinal and dietary leafy African vegetables

David R. Katerere1*, Giulia Graziani2, Kaizer M. Thembo1,3, Norman Z. Nyazema3 and Alberto Ritieni2    
1Medical Research Council, PROMEC Unit, Francie van Zijl Drive, Tygerberg, Cape Town 7505, South Africa. 2University of Naples Federico II, Facoltà di Farmacia, Dipartimento di Chimica Farmaceutica e Tossicologica, via Domenico Montesano 49 80100, Napoli, Italy. 3Department of Pharmacy, Faculty of Health Sciences, University of Limpopo, Turfloop Campus, Sovenga, South Africa.    
Email: [email protected]

  •  Accepted: 03 February 2012
  •  Published: 28 February 2012

Abstract

Seven medicinal and dietary plant species from Southern Africa were analysed for their antioxidant and total phenolic content. These were Lippia javanica, Tagetes minuta, Bidens pilosa, Vigna unguiculata, Amaranthus spinosus, Telfairia occidentalisand Corchorus olitarius. Aqueous methanol extracts were tested for free radical scavenging and anti-oxidant activity using three standard assays including 2,2′azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant potential (FRAP). The Folin-Ciocalteu assay was used to determine the total phenolic content with gallic acid as a standard. The antioxidant activity of the plants ranged from 0.76 to 5.77 mmol TEAC/100 g (ABTS), 16.29 to 1711.22 mmol TEAC/100 g (DPPH) and 0.58 to 6.12 mmol TEAC/100 g (FRAP). B. pilosa and C. olitarius had the best activity in all assays, while V. unguiculata and A. spinosus were the least active. The total phenolic content ranged from 19.79 to 333.56 mg gallic acid equivalent (GAE)/100 g. In general, there was a good correlation between antioxidant activity and total phenolic content. These results imply that these plant species may possess health promoting effects and might be potential sources of potent natural antioxidants.

 

Key words: Dietary plants, metabolic stress, antioxidant activity, total phenolic content.

 

 

Abbreviation

 

ABTS, 2,2′Azinobis-3-ethylbenzthiazoline-6-sulphonic acid; DPPH,1,1-diphenyl-2-picrylhydrazyl; FRAP, ferric reducing antioxidant potential; GAE, gallic acid equivalen