Abstract

The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production.Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was also performed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability of plant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding for semidwarfism in traditional rice varieties.

Key words: Rice, semidwarfism, sd1, environmental influence.

Abbreviation

MAB, Marker assisted backcrossing; SSR, simple sequence repeat or microsatellites markers; RPG, recurrent parent genome; ANCOVA,analysis of covariance; IRRI, international rice research institute; DGWG, dee-geo-woo-gen; sd1, semidwarf 1 gene; RFLP, restriction fragment length polymorphism;RPG, recurrent parent genome; CO, COTArroz; BB, bico da barca; GA, gibberellic acid; L/B, length to breadth ratio; SES, standard evaluation system; PCR,polymerase chain reaction;  CTAB, cetyltrimethyl ammonium bromide; DMSO,dimethyl sulfoxide; SD, standard deviation; M, mean; QTLs,