April 2010
Carbon source feeding strategies for recombinant protein expression in Pichia pastoris and Pichia methanolica
Pichia pastoris and Pichia methanolica have been used as expression systems for the production of recombinant protein. The main problems of the production are the slow hierarchic consumption of ethanol and acetate which cause toxicity problems due to methanol accumulation when this surpasses 0.5 gl-1. In some cases, the laboratory scale cultures does not show the methanol accumulation problems...
April 2010
cDNA, genomic sequence cloning and overexpression of ribosomal protein S16 gene (RPS16) from the Giant Panda
RPS16 of eukaryote is a component of the 40S small ribosomal subunit encoded byRPS16 gene and is also a homolog of prokaryotic RPS9. The cDNA and genomic sequence of RPS16 was cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription-polymerase chain reaction (RT-PCR) technology and Touchdown-PCR, respectively, which were both sequenced...
April 2010
Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study
The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production.Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to...
April 2010
The in vivo effect of N-nitrosomorpholine on the activity of enzymes in rat blood serums and liver
Neoplasm antigens outnumber the enzymes which are utilized to determine the cancer. Cancer development in the living organisms chronologically follows the cytotoxic, organotoxic and mutagenic alterations. Generally, the first symptom for chemical carcinogens is a metabolical response in connection with the detoxification phenomenon and for the infective agents the first symptom is often an immune response. Many...
April 2010
Molecular characterization and expression of DgZFP1, a gene encoding a single zinc finger protein in chrysanthemum
A single zinc finger protein gene was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach and was designated as DgZFP1. TheDgZFP1 encodes a protein of 168 amino acids residues with a calculated molecular mass of 18.1 kDa and theoretical isoelectric point is 4.71. DgZFP1...
April 2010
Influence of genotype and age of explant source on the capacity for somatic embryogenesis of two Cavendish banana cultivars (Musa acuminata Colla, AAA)
The embryogenic capacities of flower explants from one- and two-week-old male inflorescence buds from Musa acuminata Cavendish, AAA, genotypes ‘Williams’ and ‘Grand Naine’ were investigated. Explants of hands with immature flowers were excised and induced for embryogenesis. Highly significant differences were found in the embryogenic response, depending on the genotype and the...
April 2010
Study on the association of BoLA-DRB3.2 alleles with clinical mastitis in Iranian Holstein and Sarabi (Iranian native) cattle
Seventy-five Iranian Holstein cattle which comprised of two different groups (49 susceptible cattle to clinical mastitis and 26 resistant cattle to clinical mastitis) as well as forty-five Sarabi breed cattle were studied randomly for polymorphism in BoLA-DRB3.2 gene. Genotyping by single strand conformation polymorphism- polymerase chain reaction (SSCP-PCR) and then sequencing revealed the presence of previously...
April 2010
High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra
A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these...
April 2010
Growing degree day and sunshine radiation effects on peanut pod yield and growth
Growth and development of peanut (Arachis hypogaea L.) are affected by different uncontrollable environmental conditions. The objective of this work was to investigate the effect of different planting dates, thermal temperatures (growing degree days, GDD) and daily sunshine duration on morphological and agronomic traits of three commercial peanut cultivars (Gazipasa, Florispan, and NC-7) and a local cultivar....
April 2010
Growth rhythms in sunflower (Helianthus annuus L.) in response to environmental disparity
Sunflower hybrids maintain high level of stability in a variety of environments. Environmental variations affect and modify plant attributes like growth, development and assimilation through physio-morphic functions, thus modifying plant phenology. Field experiments, one each in spring and autumn, were conducted at Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi, Pakistan for two years (2007 and 2008) to...
April 2010
Production of polyploids from cultured shoot tips of Eucalyptus globulus Labill by treatment with colchicine
Polyploids from cultured shoot tips of Eucalyptus globulus were produced by treatment with colchicine. Results showed that the combination of 0.5% colchicine and treating multiple shoot clumps for 4 days was the most appropriate conditions for E. globulus polyploidy induction and the effect of the use of multiple shoot clumps for colchicine polyploidy-induced was better than single...
April 2010
Cloning and differential expression of 1-aminocyclopropane-1-carboxylate synthase gene in different floral tissues of Dendrobium ‘anna’ flowers
Cloning of the DenACS from Dendrobium hybrid cultivar Anna was performed by RT-PCR and nucleotide sequence analysis revealed that the open reading frame of this gene was 1,308 bp in length, encoded for a protein of 435 amino acid residues. The calculated molecular mass of the deduced polypeptide is 48.5 kDa and the predicted isoelectric point is 5.84. The deduced amino acid sequence of...
April 2010
The effects of sowing time and depth on germination and seedling percentage of the Taurus Cedar (Cedrus libani A. Rich.)
The aim of this study is to determine the appropriate sowing time and depth in spring for Taurus Cedar (Cedrus libani A. Rich) in Turkey. The effects of sowing time and depth were determined with regard to the germination rate of seedlings’ quality. The seeds were collected from Kapidag-Isparta, in Turkey, in 2003 and 2004. The seeds were sown at 6 different dates and 5 different depths under natural...
April 2010
Effect of dietary vitamin E supplementation on lipid and colour stability of chicken thigh meat
Lipid and myoglobin oxidation are major causes of meat quality deterioration during storage of fresh chicken meat. Our objective is to determine the effects of dietary α-tocopherol supplementation on lipid and colour stability of fresh chicken thigh meat exposed in a supermarket shopwindow or stored in a refrigerator. Chickens were fed with diets containing 25 (control), 100, 200 or 300 mg vitamin E/kg of food for...
April 2010
Detection and identification of Staphylococcus aureus in raw milk by hybridization to oligonucleotide microarray
Staphylococcus aureus causes foodborne diseases if consumed in contaminated milk products. Rapid detection and characterization of foodborne pathogen S. aureus is crucial for epidemiological investigations and food safety surveillance. It is still a challenge to detect and identify bacterial pathogens quickly and accurately according to the samples. In this study, we have amplified 16S rRNA of S....
April 2010
Supercritical carbondioxide extraction of cypermethrin in different fresh vegetables using anhydrous sodium sulfate for sample preparation and extraction
Supercritical fluid extraction (SFE) is a promising method of extraction for pesticide residues in fruits and vegetables. A rapid and high percetage of recoveries extraction of cypermethrin insecticides from fresh yardlong bean, carrot and eggplant vegetables matrixs using drying agent and SC-CO2 was developed in this study. In vegetable samples, which typically...
April 2010
Serum protein fingerprint of patients with gastric cancer by SELDI technology
To study the serum protein fingerprint of patients with gastric cancer and to screen for protein molecules closely related to gastric cancer during the onset and progression of the disease using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Serum samples from 80 gastric cancers and 80 healthy volunteers. WCX2 protein chip and PBSII-C protein chips reader (Ciphergen...
April 2010
Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
Madagascar periwinkle is a common decorative, easy growing and spreading perennial herb. Phyllody, virescence, proliferation, little leaf and yellowing symptoms were observed on periwinkle in Serdang and Banting, Selangor, Malaysia. Polymerase chain reaction (PCR) assays using P1/P7 universal phytoplasma primers and secA primers designed for identification and classification of phytoplasma, amplified 1.8 kb fragment...
April 2010
Studies on anti-ulcer, analgesic and antipyretic properties of the ethanolic leaf extract of Gongronema latifolium in rodents
The ethanol extract of Gongronema latifolium leaves were evaluated for anti-ulcer, analgesic and anti pyretic activities in rats and mice. Ethanol-induced gastric ulceration, acetic acid-induced writhing and formalin-induced nociception were used. Yeast-induced hyperpyrexia was used to investigate the antipyretic activity. The extract produced a significant ulcer protective activity in rats. The extract also...
April 2010
Antibacterial activity and composition of the essential oils of two endemic Salvia sp. from Turkey
Water-distilled essential oils from dried aerial parts of Salvia cryptantha and Salvia heldreichiana (Lamiaceae), endemic in Turkey, were analysed using gas chromatography-mass spectrometry (GC-MS). Camphor (19.1%), 1,8-cineole (16.4%), borneol (11.9%), viridiflorol (11.5%) and bornyl acetate (2.4%) were found to be the major constituents in the oil of S. cryptantha. The major constituents in...
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