African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Table of Content: 12 April 2010; 9(15)

April 2010

Carbon source feeding strategies for recombinant protein expression in Pichia pastoris and Pichia methanolica

Pichia pastoris and Pichia methanolica have been used as expression systems for the production of recombinant protein. The main problems of the production are the slow hierarchic consumption of ethanol and acetate which cause toxicity problems due to methanol accumulation when this surpasses 0.5 gl-1. In some cases, the laboratory scale cultures does not show the methanol accumulation problems...

Author(s): Raúl A. Poutou-Piñales, , Henry A. Córdoba-Ruiz, Luis A. Barrera-Avellaneda and Julio M. Delgado-Boada

April 2010

cDNA, genomic sequence cloning and overexpression of ribosomal protein S16 gene (RPS16) from the Giant Panda

RPS16 of eukaryote is a component of the 40S small ribosomal subunit encoded byRPS16 gene and is also a homolog of prokaryotic RPS9. The cDNA and genomic sequence of RPS16 was cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription-polymerase chain reaction (RT-PCR) technology and Touchdown-PCR, respectively, which were both sequenced...

Author(s): Wan-ru Hou, Yi-ling Hou, Yan-zhe Hao,Tian Zhang, Zheng-song Peng, Yan-Song and Guang-fu Wu

April 2010

Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study

The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production.Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene allowed designing a more precise breeding strategy - marker assisted backcrossing (MAB) - to...

Author(s): Sónia Negrão, Jayamani Palaniappan, João Maroco, Tiago Lourenço, David Mackill and Maria Margarida Oliveira

April 2010

The in vivo effect of N-nitrosomorpholine on the activity of enzymes in rat blood serums and liver

Neoplasm antigens outnumber the enzymes which are utilized to determine the cancer. Cancer development in the living organisms chronologically follows the cytotoxic, organotoxic and mutagenic alterations. Generally, the first symptom for chemical carcinogens is a metabolical response in connection with the detoxification phenomenon and for the infective agents the first symptom is often an immune response. Many...

Author(s): M. Maras

April 2010

Molecular characterization and expression of DgZFP1, a gene encoding a single zinc finger protein in chrysanthemum

A single zinc finger protein gene was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach and was designated as DgZFP1. TheDgZFP1 encodes a protein of 168 amino acids residues with a calculated molecular mass of 18.1 kDa and theoretical isoelectric point is 4.71. DgZFP1...

Author(s): Liu Qinglin, Xu Kedong, Zeng Li, Ma Nan and Zhao Liangjun

April 2010

Influence of genotype and age of explant source on the capacity for somatic embryogenesis of two Cavendish banana cultivars (Musa acuminata Colla, AAA)

The embryogenic capacities of flower explants from one- and two-week-old male inflorescence buds from Musa acuminata Cavendish, AAA, genotypes ‘Williams’ and ‘Grand Naine’ were investigated. Explants of hands with immature flowers were excised and induced for embryogenesis. Highly significant differences were found in the embryogenic response, depending on the genotype and the...

Author(s): Muhammad Youssef, Andrew James, Alberto Mayo-Mosqueda, José Roberto Ku-Cauich, Rosa Grijalva-Arango and Rosa María Escobedo-GM

April 2010

Study on the association of BoLA-DRB3.2 alleles with clinical mastitis in Iranian Holstein and Sarabi (Iranian native) cattle

Seventy-five Iranian Holstein cattle which comprised of two different groups (49 susceptible cattle to clinical mastitis and 26 resistant cattle to clinical mastitis) as well as forty-five Sarabi breed cattle were studied randomly for polymorphism in BoLA-DRB3.2 gene. Genotyping by single strand conformation polymorphism- polymerase chain reaction (SSCP-PCR) and then sequencing revealed the presence of previously...

Author(s): Masomeh Firouzamandi, Jalil Shoja, Abolfazl Barzegari and Esmaeil Roshani

April 2010

High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra

A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these...

Author(s): Esin Akçam Oluk, Serpil Orhan, Özgür Karakaş, Ali Çakır, and Ahmet Gönüz

April 2010

Growing degree day and sunshine radiation effects on peanut pod yield and growth

Growth and development of peanut (Arachis hypogaea L.) are affected by different uncontrollable environmental conditions. The objective of this work was to investigate the effect of different planting dates, thermal temperatures (growing degree days, GDD) and daily sunshine duration on morphological and agronomic traits of three commercial peanut cultivars (Gazipasa, Florispan, and NC-7) and a local cultivar....

Author(s): Öner Canavarand Mustafa Ali Kaynak

April 2010

Growth rhythms in sunflower (Helianthus annuus L.) in response to environmental disparity

Sunflower hybrids maintain high level of stability in a variety of environments. Environmental variations affect and modify plant attributes like growth, development and assimilation through physio-morphic functions, thus modifying plant phenology. Field experiments, one each in spring and autumn, were conducted at Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi, Pakistan for two years (2007 and 2008) to...

Author(s): Shuaib Kaleem, Fayyaz- ul- Hassan, Abdul Razzaq, Abdul Manaf and Aamir Saleem

April 2010

Production of polyploids from cultured shoot tips of Eucalyptus globulus Labill by treatment with colchicine

Polyploids from cultured shoot tips of Eucalyptus globulus were produced by treatment with colchicine. Results showed that the combination of 0.5% colchicine and treating multiple shoot clumps for 4 days was the most appropriate conditions for E. globulus polyploidy induction and the effect of the use of multiple shoot clumps for colchicine polyploidy-induced was better than single...

Author(s): H. Lin, M. Jian, L. Y. Liang, W. J. Pei, X. Z. Liu and H. Y. Zhang

April 2010

Cloning and differential expression of 1-aminocyclopropane-1-carboxylate synthase gene in different floral tissues of Dendrobium ‘anna’ flowers

Cloning of the DenACS from Dendrobium hybrid cultivar Anna was performed by RT-PCR and nucleotide sequence analysis revealed that the open reading frame of this gene was 1,308 bp in length, encoded for a protein of 435 amino acid residues. The calculated molecular mass of the deduced polypeptide is 48.5 kDa and the predicted isoelectric point is 5.84. The deduced amino acid sequence of...

Author(s): Titiporn Nagtong, Sudarat Thanonkeo, Preekamol Klanrit and Pornthap Thanonkeo

April 2010

The effects of sowing time and depth on germination and seedling percentage of the Taurus Cedar (Cedrus libani A. Rich.)

The aim of this study is to determine the appropriate sowing time and depth in spring for Taurus Cedar (Cedrus libani A. Rich) in Turkey. The effects of sowing time and depth were determined with regard to the germination rate of seedlings’ quality. The seeds were collected from Kapidag-Isparta, in Turkey, in 2003 and 2004. The seeds were sown at 6 different dates and 5 different depths under natural...

Author(s): Süleyman Gülcü, H. Cemal Gültekin and Zafer Ölmez

April 2010

Effect of dietary vitamin E supplementation on lipid and colour stability of chicken thigh meat

Lipid and myoglobin oxidation are major causes of meat quality deterioration during storage of fresh chicken meat. Our objective is to determine the effects of dietary α-tocopherol supplementation on lipid and colour stability of fresh chicken thigh meat exposed in a supermarket shopwindow or stored in a refrigerator. Chickens were fed with diets containing 25 (control), 100, 200 or 300 mg vitamin E/kg of food for...

Author(s): Nacim Zouari, Fatma Elgharbi, Nahed Fakhfakh, Abir Ben Bacha, Youssef Gargouri and Nabil Miled

April 2010

Detection and identification of Staphylococcus aureus in raw milk by hybridization to oligonucleotide microarray

Staphylococcus aureus causes foodborne diseases if consumed in contaminated milk products. Rapid detection and characterization of foodborne pathogen S. aureus is crucial for epidemiological investigations and food safety surveillance. It is still a challenge to detect and identify bacterial pathogens quickly and accurately according to the samples. In this study, we have amplified 16S rRNA of S....

Author(s): Yang He, , Honglu Liu, Mengjun Xian and Yufeng Li

April 2010

Supercritical carbondioxide extraction of cypermethrin in different fresh vegetables using anhydrous sodium sulfate for sample preparation and extraction

Supercritical fluid extraction (SFE) is a promising method of extraction for pesticide residues in fruits and vegetables. A rapid and high percetage of recoveries extraction of cypermethrin insecticides from fresh yardlong bean, carrot and eggplant vegetables matrixs using drying agent and SC-CO2 was developed in this study. In vegetable samples, which typically...

Author(s): Mohammad Arifur Rahman, Dzolkhifli Omar, I. S. Md. Zaidul, M. M. Morshed and K. N. H. Bin Rashid

April 2010

Serum protein fingerprint of patients with gastric cancer by SELDI technology

To study the serum protein fingerprint of patients with gastric cancer and to screen for protein molecules closely related to gastric cancer during the onset and progression of the disease using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Serum samples from 80 gastric cancers and 80 healthy volunteers. WCX2 protein chip and PBSII-C protein chips reader (Ciphergen...

Author(s): Chibo Liu, Haibao Wang, Chunqin Pan, Sihua Mou and Yong Liang

April 2010

Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia

Madagascar periwinkle is a common decorative, easy growing and spreading perennial herb. Phyllody, virescence, proliferation, little leaf and yellowing symptoms were observed on periwinkle in Serdang and Banting, Selangor, Malaysia. Polymerase chain reaction (PCR) assays using P1/P7 universal phytoplasma primers and secA primers designed for identification and classification of phytoplasma, amplified 1.8 kb fragment...

Author(s): Naghmeh Nejat, Kamaruzaman Sijam, Siti Nor Akmar Abdullah, Ganesan Vadamalai and Matthew Dickinson

April 2010

Studies on anti-ulcer, analgesic and antipyretic properties of the ethanolic leaf extract of Gongronema latifolium in rodents

The ethanol extract of Gongronema latifolium leaves were evaluated for anti-ulcer, analgesic and anti pyretic activities in rats and mice. Ethanol-induced gastric ulceration, acetic acid-induced writhing and formalin-induced nociception were used. Yeast-induced hyperpyrexia was used to investigate the antipyretic activity. The extract produced a significant ulcer protective activity in rats. The extract also...

Author(s): Akuodor G. C., Idris-Usman M. S., Mbah, C. C., Megwas U. A., Akpan, J. L., Ugwu T. C., Okoroafor D. O. and Osunkwo U. A.

April 2010

Antibacterial activity and composition of the essential oils of two endemic Salvia sp. from Turkey

Water-distilled essential oils from dried aerial parts of Salvia cryptantha and Salvia heldreichiana (Lamiaceae), endemic in Turkey, were analysed using gas chromatography-mass spectrometry (GC-MS). Camphor (19.1%), 1,8-cineole (16.4%), borneol (11.9%), viridiflorol (11.5%) and bornyl acetate (2.4%) were found to be the major constituents in the oil of S. cryptantha. The major constituents in...

Author(s): Mehtap Akin, Betul Demirci, Yavuz Bagci and K. Husnu Can Baser