African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Microbial transformation of neomycin by a mutant of neomycin-producing Streptomyces fradiae

Lin Ma1, Lingyun Yang1, Yun Liu1, Guanying Shi1, Congjun Yang1, Chunyu Zhou2, Weichang Xu2, Ke Tao1*, Taiping Hou1*  
  1College of Life Sciences, Key Laboratory of Bio-Resource and Eco-environment, Ministry of Education, Sichuan University, Chengdu 610064, P. R. China. 2Shenyang Research Institute of Chemical Industry, Shenyang 110021, P. R. China.
Email: [email protected], [email protected]

  •  Accepted: 21 September 2010
  •  Published: 06 December 2010



Utilizing a mutant of neomycin-producing Streptomyces fradiae mutagenized with neutron radiation, biotransformation of neomycin into modified compounds was studied. The biotransformation products were isolated by ion exchange chromatography and monitored by thin layer chromatography bioautography (TLCB). Antibacterial activity of biotransformation products against ten species of bacteria including four plant pathogens was tested qualitatively by TLCB and detected quantitatively by Oxford cup method. The minimal inhibitory concentration (MIC) of biotransformation products was tested by agar diffusion method. Three isolated transformation products had obvious antibacterial activity againstStaphylococcus aureusBacillus subtilisProteus vulgaris and Pseudomonas solanacarum. At the concentration of 50 g/ml, the transformation product X had a similar antibacterial effect with neomycin but the transformation product Y and Z showed a decreased effect compared to neomycin except for P. vulgaris and P. solanacarum. However, the results from MIC analysis demonstrated that only the transformation product X maintained the same inhibitory effect with neomycin.


Key words: Neomycin, biotransformation, Streptomyces fradiae, mutant, neutron radiation.