African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Chemical fingerprint technique and its application in the classification and quality assessment of the Gastrodia tuber

Jun Tao1,3*, Chenqin Qian2, Zhongqiu Tang3, Ping Chen1, Yan Wang1 and Yunrui Han1
1School of chemistry and bioengineering, Changsha University of Science and Technology, Changsha, 410076, P. R. China. 2Guangdong Hanses Pharmaceutical Technology Co., Ltd. Guangzhou, 510335, P. R. China. 3Huayuan County Government, Hunan province, 416400, P. R. China.
Email: [email protected]

  •  Accepted: 10 June 2011
  •  Published: 23 November 2011

Abstract

The Gastrodia tuber and its active component, gastrodin, have many pharmacological effects. In this study, optimized high performance liquid chromatography (HPLC) parameters were employed to determine the chemical fingerprints and gastrodin content of nine Gastrodia tuber populations. Based on the degree of similarity of the chemical fingerprints, the nine Gastrodia tuber populations were grouped into one of the three different classes. Class I Gastrodia tubers had the highest content of gastrodin and were thus, regarded as possessing the highest quality. Of the class I Gastrodia tuber samples, those from Yichang, Hubei and Shimen, Hunan, were identified as the “best.” Close relationships were detected among the chemical fingerprints, gastrodin content and place of origin of theGastrodia tubers. Hence, these findings may be applied in assessing the quality ofGastrodia tubers and in identifying and segregating poor quality Gastrodiapopulations from those of good quality.

 

Chemical fingerprint, chromatographic classification, Gastrodia tuber, gastrodin content, HPLC, quality assessment.