African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genotoxic effects of 5-bromouracil on cytomorphological characters of Cichorium intybus L.

Iram Fatma Jafri*, Ainul Haq Khan and Mohd Gulfishan
  1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia. 2School of Biological Sciences, Universiti Sains Malaysia, Minden Heights, 11800, Penang, Malaysia. 3Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia. 4Sime Darby Seeds and Agricultural Services Sdn. Bhd., KM 10, Jln. Banting-Kelanang, P. O. Box 207, 42700 Banting, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 21 July 2011
  •  Published: 12 September 2011

Abstract

 

 

This work was done to study the effect of base analogue 5-bromouracil (5-BU) on the medicinal herb, Cichorium intybus. 5-BU induced miss pairing during the DNA replication. The seeds of C. intybus were treated with different concentrations of 5-BU. Variations in some parameters such as seed germination, seedling survival, seedling height, pollen fertility, days to flowering, days to maturity, number of leaves per plant, plant height, and chromosome behavior were studied in M1 generation. A positive correlation between increasing concentrations of mutagen and various cytomorphological characters of C. intybus was observed.
 
 
 
 
Key words: Chromosomal aberrations, Cichorium intybus, morphological variations, 5-bromouracil.