September 2011
Investigation of genetic variability related to the in vitro floral hermaphrodism induction in Date palm (Phœnix dactylifera L.)
This paper reports on a molecular analysis study conducted on Date palm flowers from the Deglet Nour cultivar to investigate putative genetic variability related to thein vitro floral hermaphrodism induction. Natural male and female as well as hermaphrodite ones that were produced in vitro through the hormonal treatment of female flowers were submitted to ISSR-PCR...
September 2011
Twenty putative palmitoyl-acyl transferase genes with distinct expression patterns in Arabidopsis thaliana
Palmitoylation is a reversible posttranslational addition of palmitate to cysteine residues in proteins through a thioester bond by a family of DHHC (Asp-His-His-Cys) palmitoyltransferases (PATs) involved in cellular signaling, membrane trafficking, and synaptic transmission. There are 20 genes containing DHHC domain predicted to encode putative palmitoyltransferase in Arabidopsis thalianagenome....
September 2011
Nitrogen remobilization in wheat as influenced by nitrogen application and post-anthesis water deficit during grain filling
Pre-anthesis stored nitrogen in wheat (Triticum aestivum L.) is important because grain filling greatly depends on the remobilization of pre-anthesis nitrogen under Mediterranean growth conditions. This field study was conducted to assess the effect of post-anthesis water deficit and three N fertilizer levels on N remobilization and N losses in three wheat cultivars (‘Shiraz’,...
September 2011
Genotoxic effects of 5-bromouracil on cytomorphological characters of Cichorium intybus L.
This work was done to study the effect of base analogue 5-bromouracil (5-BU) on the medicinal herb, Cichorium intybus. 5-BU induced miss pairing during the DNA replication. The seeds of C. intybus were treated with different concentrations of 5-BU. Variations in some parameters such as seed germination, seedling survival, seedling height, pollen fertility, days to flowering, days to maturity,...
September 2011
Studies on new antifreeze protein from the psychrophilic diatom, Fragilariopsis cylindrus
Antifreeze proteins (AFPs) are found in a wide range of species including fishes, plants, etc. They have very characteristic feature that inhibit the growth and recrystallization of ice that forms in intercellular spaces. Two expressed sequence tags (ESTs) were previously identified from salt stress cDNA library inFragilariopsis cylindrus to have similarities with snow mold AFP. Using bioinformatics...
September 2011
Induced accumulation of 20-hydroxyecdysone in cell suspension cultures of Vitex glabrata R.Br.
This study describes the effects of culture medium, culture temperature, sucrose concentration and cholesterol feeding on cell growth and 20-hydroxyecdysone production in suspension cultures of Vitex glabrata, an important medicinal plant in Thailand. Cell growth and 20-hydroxyecdysone production were not significantly different when cells were cultivated on B5 or half-strength MS medium. However,...
September 2011
Reduced loss of NH3 by coating urea with biodegradable polymers, palm stearin and selected micronutrients
In agricultural lands, the loss of NH3 from surface-applied urea and micronutrient deficiencies are the two most common problems, which can be solved by using coated urea with micronutrients and biodegradable natural materials. These coatings can improve the nutrient status in the soil and simultaneously reduce nitrogen loss from urea. To control ammonia loss and urea’s hydrolysis process, two...
September 2011
The induction and growth of potato (Solanum tuberosum. L) microtubers (sante cultivar) in response to the different concentrations of 6-benzylaminopurine and sucrose
The effects of different concentrations of 6-benzylaminopurine (BAP) and sucroseas induction combinations on microtuber formation and the time of this process, numbers and fresh and dry weight of microtubers were investigated. A two-stage culture was used to study the effect of hormonal and sucrose treatments. In the first stage, a liquid MS medium containing 0.5 mgl-1 BAP + 0.4 mgl-1 gibberellic...
September 2011
Variability salt stress response analysis of Tunisian natural populations of Medicago truncatula (Fabaceae) using salt response index (SRI) ratio
We evaluated the responses to salt stress of 106 Medicago truncatula lines from 11 Tunisian natural populations collected from areas that varied in soil composition, salinity and water availability. Five references lines were also included in this study. Plants were cultivated in two treatments (0 and 50 mM of NaCl) during a period of 60 days. At harvest, we measured 14 quantitative traits of the...
September 2011
Isolation and characteristic of an aerobic denitrifier with high nitrogen removal efficiency
Paracoccus denitrificans DL-23, isolated from aerobic domesticated activated sludge, was demonstrated to have high ability of denitrification and heterotrophic nitrification under aerobic condition. After optimization (succinate, COD/N 10, 37°C, 160 rpm), DL-23 removed 420 and 860 mg/l NO3- -N within 36 to 60 h of growth, respectively. DL-23 also removed 380 mg/l NH4+-N within 24 h with ammonia...
September 2011
Antagonistic effect of Trichoderma harzianum VSL291 on phytopathogenic fungi isolated from cocoa (Theobroma cacao L.) fruits
In this study we evaluated the antagonism in vitro of Trichoderma harzianum strain VSL291 against 18 pathogens of cocoa fruits in dual culture. T. harzianum VSL291 inhibited the growth of the phytopathogenic fungi tested between 10.54 and 85.43%. The mycoparasitism of Moniliophthora roreri by T. harzianum VSL291 was studiedby light and scanning...
September 2011
Bioleaching of copper, aluminum, magnesium and manganese from brown shale by Ganoderma lucidum
The present study was done to check the bioleaching feasibility of brown shale for the recovery of copper (Cu), aluminum (Al), magnesium (Mg) and manganese (Mn) ions using Ganoderma lucidum. Different experimental parameters were optimized for the enhanced recovery of metals ions. Effect of different substrates like glucose, molasses, saw dust and cotton seed cake on the recovery of metals ions was...
September 2011
Bioaccumulation of heavy metals by Dyera costulata cultivated in sewage sludge contaminated soil
High concentrations of heavy metals are harmful to plants, animals and humans and their potential accumulation in human tissues and bio-magnification through the food chain cause serious health hazards. An experiment was conducted in the glasshouse to evaluate the potential of Dyera costulata as a bioaccumulator to absorb heavy metals from sewage sludge contaminated soils. D. costulataseedlings...
September 2011
Application and comparison of two different intraspecific protoplast fusion methods in Trichoderma harzianum and their effect on β-glucosidase activity
In an attempt to construct superior Trichoderma harzianum isolates for improving β-glucosidase productivity, protoplast fusion technique was applied. After application of different mutagenic treatments, twenty mutants were chosen to be tested for their resistance or sensitivity against four antifungal agents. Out of them, four isolates were selected on the basis of their response to antifungal...
September 2011
Ethanol production from Jerusalem artichoke (Helianthus tuberosus L.) by Zymomonas mobilis TISTR548
The selection and characterization of Zymomonas mobilis for ethanol production from Jerusalem artichoke (Helianthus tuberosus L.) juice was investigated. Growth and ethanol production of four Z. mobilis strains isolated in Thailand, that is, TISTR 405, TISTR 548, TISTR 550 and TISTR 551, were compared with those of the type strain Z. mobilis ZM4 (NRRL B-14023) at...
September 2011
Production and antioxidative activity of alcoholic beverages made from Thai ou yeast and black rice (Oryza sativa var. Indica cv. Shiun)
Fermentation yeast was isolated from a Thai traditional alcoholic beverage called Thai ou, which is drunk through bamboo tubes. The isolated yeast was identified as a strain of the genus Saccharomyces cerevisiae. The alcoholic beverage made with the isolated yeast designated as S. cerevisiae NP01 from black rice grains had an ethanol concentration of 12.4 to 13.1% (v/v) and a large amount of phenolic...
September 2011
Endothelial nitric oxide synthase gene Glu298Asp polymorphism and risk of preeclampsia in South East of Iran
Preeclampsia (PE) is the most serious complication of pregnancy that causes maternal and fetal morbidity and mortality. Although the exact pathophysiology of PE is unknown, a large number of studies have shown that abnormalities in nitric oxide (NO) synthesis may contribute to the development of this disorder. There are some evidences that polymorphisms of the endothelial nitric oxide synthase (eNOS) gene...
September 2011
Effect of different levels of royal jelly on biochemical parameters of swimmers
This study aims to investigate the effects of different levels of royal jelly supplementation on biochemical parameters in swimmers. Randomly selected 40 male swimmers aged 18 to 25 years attending the same trainings were recruited. Swimmers were assigned to 4 groups each with 10 subjects. Varying amounts of royal jelly (2, 1 g and 500 mg) were given to the 1st, 2nd and 3rd groups and placebo (corn starch) to...
September 2011
Iron content in forage sorghum (Sorghum bicolor (L.) Moench) measured on different slit widths with atomic absorption spectrometry
Our objective was to know the right slit width for iron (Fe) concentration of forage sorghum, sorghum hybrid (Sorghum bicolor (L.) Moench), and also to discern which water treatment sludge (WTS) were good for ruminant's health with the feeding sorghum on the present study. The present experiment was carried out on a randomized block design with four treatments; Control, alum sludge compost,...
September 2011
Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment
Traditional medicine has a key role in health care worldwide. Obtaining scientific information about the efficacy and safety of the wild plants grown in western Mediterranean coast of Egypt is one of our research goals. In this study, 10 wild plants namely Mesembryanthemum crystallinum, Blackiella aellen, Arthrocnemon glaucum, Atriplex halimus, Thymelaea...
September 2011
Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression
Rhaphidophora korthalsii methanol extract was previously found as a potential in vitro immunomodulating agent. In this study, the in vitro immunomodulatory activity of R. korthalsii methanol extract on mice splenocyte were quantified through evaluating T/NK cell population, cytokine expression (IL-2 and IFN-γ) and cytotoxicity against Yac-1 cell. In...
September 2011
Seroprevalence of human T-cell lymphotropic virus type 1 infection (HTLV1) in different patients in the north of Iran
Human T-cell lymphotropic virus type-1 (HTLV1) is a lymphotropic virus which can contribute to carcinogenesis in adult T-cell leukemia, myleopathy and other disorders. 20 million people are affected by this virus in the world. The aim of this study was to determine the incidence of human T-cell lymph tropic virus type 1 infection in patients referred to Emam’s hospital laboratory in Sari. A...
September 2011
Differentiation potential of human suspension mononucleated peripheral blood cells
Stem cells are known to have the ability to renew themselves and differentiate into a diverse range of specialized cell types. Currently, the differentiation potential of human peripheral blood mononucleated cells in suspension as stem cells is not well-understood. The aim of this study was to investigate the differentiation potential of suspension mononucleated cells from human peripheral blood to...
September 2011
Proximate composition of Mystus bleekeri in relation to body size and condition factor from Nala Daik, Sialkot, Pakistan
Proximate composition of small catfish, Mystus bleekeri, from Nala Daik, Sialkot, Pakistan was investigated and fluctuation in relation to body size and condition factor was carried out. Mean percentages for water, fat, protein and ash contents inthe whole wet body weight of wild M. bleekeri were 77.87, 3.26, 15.01 and 3.87%, respectively. Body composition of the fish was...
September 2011
Initial requirements for embryogenic calluses initiation in thin cell layers explants from immature female oil palm inflorescences
This study highlights procedures for embryogenic calluses induction from immature female inflorescences of oil palm using thin-cell-layers explants (TCL). In three experiments, the ability of calluses induction were examined and identified through different types of basal media, position of the TCL explants in the rachillae and concentrations of 2,4-D and types of antioxidants added into the...
September 2011
Analysis of the effects of cerium on calcium ion in the protoplasts of Arabidopsis thaliana with confocal microscopy
The laser-scanning confocal microscopy has become a routine technique and indispensable tool for cell biological studies. In this study, the probe Fluo-3 AM was used to research the instantaneous changes of calcium ion (Ca2+) in the protoplasts of Arabidopsis thaliana. The laser-scanning mode of confocal microscope is XYT, and the time interval between two images is 10.635 s. 30optical sections were...
September 2011
Improved secretory production of calf prochymosin by codon optimization and disruption of PMR1 in Kluyveromyces lactis GG799
Chymosin as an important industrial enzyme used widely in cheese manufacture. In our preliminary study, low yields (80 U mL-1) were obtained when Kluyveromyces lactis GG799 was used to express chymosin. We investigated whether this poor secretion could be improved by codon optimization and disruption of PMR1 gene inK. lactis GG799. Our study shows that combination of codon...
September 2011
Efficacy of Avicennia marina (Forsk.) Vierh. leaves extracts against some atmospheric fungi
Crude ethanolic extract of Avicennia marina leaves was tested against seven allergenic fungi viz., Alternaria alternata, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium herbarum, Penicillium notatum andSaccharomyces cerevisiae using five different solvents: dimethyl sulphoxide(DMSO), distilled water (DW), chloroform, ethanol and acetone at 2000, 4000...
September 2011
An in vitro evaluation of antibacterial effect of silver nanoparticles on Staphylococcus aureus isolated from bovine subclinical mastitis
Bovine mastitis is the most costly disease affecting dairy cows and milk production. As Staphylococcus aureus is considered as a major pathogen due to its prevalence in dairy herds, contagious nature of infection, economic impact of treatment and control and its resistance to antimicrobial agents is a well-documented challenge in dairy cows. Therefore, the purpose of this study was to...
September 2011
Impact of new synthesized analogues of dehydroacetic acid on growth rate and vomitoxin accumulation by Fusarium graminearum under different temperatures in maize hybrid
Previous work indicated that some of the new synthesized analogues of dehydroacetic acid (DHA) were inhibitory to the growth of mycotoxin producing moulds and accumulation of aflatoxin B1 (AFB1) and ochratoxin A (OTA). The objective of this study was to determine the specific new synthesized chemical compounds that may be effective against mould growth and vomitoxin (deoxynivalenol) (DON) accumulation...
September 2011
Effect of calcium salts and tri-sodium citrate on certain properties of calcium-fortified soy milk
Soybean milk was fortified with calcium salts to increase its calcium contents and improve its nutritional value. The addition of calcium fortifiers gave rise to a significant drop of pH and a marked rise of calcium ion concentration. Addition of calcium salts to soy milk had a pronounced negative effect on its heat stability against heat-induced and calcium-induced coagulation. Tri-sodium citrate was added to improve...
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