African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Comparison of certain microbial counting methods which are currently commonly used in the soaking process

Eser Eke BayramoÄŸlu1, Gonca Telli Yamamoto2* and Düden Uluç3
  1Department of Leather Engineering, Faculty of Engineering Ege University 35100 Bornova-Izmir, Turkey 2Department of Information Systems, School of Applied Sciences, Okan University 34959 Tuzla/Istanbul, Turkey. 3Department of Leather Engineering, Faculty of Engineering Ege University 35100 Bornova-Izmir, Turkey.
Email: [email protected]

  •  Accepted: 23 November 2009
  •  Published: 15 December 2009

Abstract

 

This study examines the interrelation of the test methods which are commonly applied in the leather industry for hide or skin soaking. For this purpose, five different bactericides were given during the soaking processes and after the presoaking process. The processes were measured at the first 20th min, 24th and 48th h. For this purpose, two different ATP test kits, dip slide and agar pour plate method were used in order to determine the microorganism load as RLU (Relative Light Units) or CFU (Colony Forming Units)/ml. The values with all methods were identified as highly different from each other, while the statistical analyses indicated positive Pearson correlation coefficients especially between the 24th and 48th h measurements. The appearance of a positive correlation between the different methods under constant conditions implied the effectiveness of the bactericides to the customer at the factory environment. The ATP test method and dip slide methods that are commonly used in the market are correct and convenient methods offered by some companies.

 

Key words: Leather industry, soaking process, bacteria number, bactericide.