African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Analysis of HMWGS of historical set of Pakistani bread wheat varieties using SDS-PAGE

Muhammad Ali 1,3,Muhammad Faisal Saddiqui2*, Ihsan Ullah 1,3,and Jehan Bakht1
1 Institute of Biotechnology and Genetic Engineering, NWFP Agricultural University, Pakistan. 2Faculty of Chemical and  Natural Resource Engineering, University of Malaysia Pahang (UMP), Kuantan, Malaysia. 3Department of Biotechnology, Sarhad University of Science and Information Technology Pakistan.
Email: [email protected]

  •  Published: 31 July 2010

Abstract

In the present study an attempt has been made to characterize thirty bread-wheat varieties of Pakistan for High Molecular Weight Glutenin Subunits (HMW- GS). Glutenin proteins form a continuous proteinaceous matrix in the cells and form a continuous viscoelastic network during the mixing process of dough development. Glutenin consists of High Molecular Weight (HMW) and Low Molecular Weight (LMW) subunits. The HMW Glutenin Subunits (HMW-GS) are chiefly vital for determining dough elasticity. The core objective of our research work was to inspect the glutenin subunits by sodium dodecyl-sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) and compare the banding pattern with Chinese Spring High-Molecular-Weight Glutenin Subunits (HMW-GS). The bands were numbered according to Payne's numbering system and varieties were accordingly assigned theoretical quality scores. All the tested varieties indicated null allele forgluA1 locus, 17 + 18 for gluB1 locus and 2 + 12 for gluD1 locus. This result indicating that all varieties have similar bread making quality alleles at HMWGS loci. The varieties containing 5 + 10 HMWGS allele at gluD1 locus have better bread making quality. Better bread making wheat varieties may be produced by crossing the local varieties of gluA1 locus, 17 + 18 for gluB1 locus and 2 + 12 for gluD1 locus with 5 + 10 HMWGS allele at gluD1 locus.

 

Key words: Wheat varieties, SDS-PAGE, HMWGS, Payne's numbering.