African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

Designing of enzyme linked immunosorbent assay (ELISA) kit for diagnosis copro-antigens of Giardia lamblia

Afshin Barazesh1*, Jafar Majidi2, Esmaeel Fallah2, Rasul Jamali2, Jalal Abdolalizade3 and Reza Gholikhani4
1Department of Microbiology and Parasitology, Faculty of Medicine, The Persian Gulf Tropical and Infectious Diseases Research Center, Bushehr University of Medical Sciences, P. O. Box 3631, Bushehr, Iran. 2Department of Immunology and Parasitology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 3Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 4Department of Biochemistry and Nutrition, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Email: [email protected]

  •  Accepted: 25 June 2010
  •  Published: 31 August 2010

Abstract

The sensitivity of microscopic examination of fecal samples to recognize Giardiaparasites is low. In the methods based on antigen scanning of parasites such as enzyme linked immunosorbent assay (ELISA), copro-antigens of parasite will be traced and diagnosed even if the live parasite is absent in the fecal samples. To design this method, a pure antibody against parasite as well as an antibody conjugated to a proper enzyme is needed. In this study, an anti-Giardia IgG extracted from serum of contaminated rabbit was purified by ion-exchange chromatography and conjugated to the enzyme horse radish peroxidase (HRP). This antibody was used to design direct and indirect ELISA kits to measure conjugation titer. In both direct and indirect ELISA methods, optical densities (ODs) were 1 by using dilution of 1/4000 of conjugation. According to the results of both tests and the success in produced conjugate, it could be proceeded to prepare ELISA kits to diagnose giardiasis infections in various samples.

 

Key words: Enzyme linked immunosorbent assay (ELISA), antibody, copro-antigen, Giardia lamblia.

Abbreviation

HRP, Horse radish peroxidase; TMB, tetra methyl benzidine; PBS,phosphate-buffered saline; ELISA, enzyme linked immunosorbent assay.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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