Abstract

GeneFishing Discovery is a system applied on disease and gene related research for eukaryotes and the starting material for the study could be either total RNA or mRNA. In this study we demonstrate the use of this discovery system for a prokaryotic system by modifying the eukaryotic protocol using the poly (A)-tailing approach. As an example we compared the gene expression pattern of two closely related species of bacteria under the same family (Burkholderia thailandensis andBurkholderia cepacia) with satisfactory results.

Key words: Genefishing, differential expression, prokaryotic system.

Abbreviation

DEG, GeneFishing Differentially Expressed Gene; PCR,polymerase chain reaction; RT-PCR, reverse transcription polymerase chain reaction; mRNA, messenger RNA; rRNA, ribosomal RNA.