African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12212

Table of Content: 30 August 2010; 9(35)

August 2010

Verification of the utility of molecular markers linked to the multiple-allele male-sterile gene Ms in the breeding of male-sterile lines of Chinese cabbage (Brassica rapa)

To verify the molecular markers linked to the genic multiple-allele male-sterile geneMs, an F1 plant, which was generated by crossing the inbred line a20 and the male-sterile plant of the genic multiple-allele male-sterile AB line, was backcrossed with an a20 plant to develop BC4 and BC5 populations. Sequence-characterized amplified region (SCAR) marker syau_scr01 and simple sequence repeat...

Author(s): Feng Hui, Wang Lili, Wei Peng, Liu Zhiyong, Li Chengyu, Wang Yugang, Ji Ruiqin and Zhang Huaqing

August 2010

Application of genefishing discovery system on differential gene expression study for prokaryotic system

GeneFishing Discovery is a system applied on disease and gene related research for eukaryotes and the starting material for the study could be either total RNA or mRNA. In this study we demonstrate the use of this discovery system for a prokaryotic system by modifying the eukaryotic protocol using the poly (A)-tailing approach. As an example we compared the gene expression pattern of two closely related species of...

Author(s): K. H. Chua, H. C. Chai and S. D. Puthucheary

August 2010

Change in amino acids content during germination and seedling growth of Cola sp.

Accessions of Cola acuminata and Cola anomala were analysed based on the variation of free amino acids during germination and seedling growth. These compounds were determined by high performance liquid chromatography (HPLC) methods. In the non-germinated seeds of C. acuminata, arginine (Arg) was the major amino acid found in all six accessions and valine (Val) was the least present, while...

Author(s): Pierre Effa Onomo, Nicolas Niemenak, Denis Omokolo Ndoumou and Reinhard Lieberei

August 2010

Investigation of ethanol productivity of cassava crop as a sustainable source of biofuel in tropical countries

The ethanol productivity of cassava crop was investigated in a laboratory experiment by correlating volumes and masses of ethanol produced to the masses of samples used. Cassava tubers (variety TMS 30555) were peeled, cut and washed. 5, 15, 25 and 35 kg samples of the tubers were weighed in three replicates, soaked in water for a period of a day, after which each sample was dried, crushed and the mash mixed with 500 ml...

Author(s): B. A. Adelekan

August 2010

An assessment of physiological workload of forest workers in felling operations

This study examined the physical strain experienced by chainsaw operator and the applicability of heart rate indices for measuring physical strain in forest felling operations in north eastern region of Turkey. The heart rate of ten chainsaw operators were recorded continuously throughout the working day and applied to heart rate indices. The average working heart rate was 122.8 beats/minute (bt. min-1), while the...

Author(s): Erhan Çalışkan and Sadık Çağlar

August 2010

Genetic linkage maps of Pinus koraiensis Sieb. et Zucc. based on AFLP markers

Genetic linkage maps provide essential information for molecular breeding. In this paper, the genetic linkage map of Pinus koraiensis was constructed using an F1 progeny of 88 individuals. One hundred and thirty (130) of molecular markers were mapped onto 6 linkage groups, 4 triples and 15 pairs at the linkage criteria LOD 4.0. Nine primer combinations were applied to map construction. The consensus map gained covers...

Author(s): Min-Min Chen, Fujuan Feng, Xin Sui and Shijie Han

August 2010

Sulphur depletion altered somatic embryogenesis in Theobroma cacao L. Biochemical difference related to sulphur metabolism between embryogenic and non embryogenic calli

Somatic embryogenesis is a useful tool for Theobroma cacao improvement and propagation. Depending on culture medium composition, different morphogenetic structures (including somatic embryo) occur in response to alteration of genes expression patterns and biochemical changes. The effect of SO42- ion deficiency in culture media on somatic embryogenesis was studied through sequential replacement of...

Author(s): Minyaka Emile, Niemenak Nicolas, Issali Emmanuel Auguste, Sangare Abdourahamane and Denis Ndoumou Omokolo

August 2010

Spatial and temporal analysis of forest cover changes in the Bartin region of northwestern Turkey

This study analyzed the changes in the forest areas in Bartin province of Turkey and the surrounding areas using remote sensing data and GIS techniques. Three Landsat Thematic Mapper (TM) images of the study region, recorded in 1987, 1992, and 2000, were utilized. The main land-use characteristics were derived using a maximum-likelihood classification technique. The remotely sensed data allows monitoring of current land...

Author(s): Ayhan Atesoglu and Metin Tunay

August 2010

Soil water distribution on different number of growing years of alfalfa pasture in the Loess Plateau of Northwest China

The alfalfa pastureland in the semiarid Loess Plateau region of Northwest China usually has dry soil layers. We studied the soil water variations on alfalfa (Medicago sativa L.) grassland with different number of growing years. Seven growing years of alfalfa grassland were chosen in this study: (1) 4-year-old, (2) 6-year-old, (3) 8-year-old, (4) 12-year-old, (5) 14-year-old, (6)18-year-old and (7) 26-year-old. The...

Author(s): Xiaolong Rena,c, Zhikuan Jiaa, Sumei Wanb and Xiaoli Chend

August 2010

Scheduling irrigation for jujube (Ziziphus jujuba Mill.)

This study was performed to select suitable indicator for scheduling the irrigation of jujube (Ziziphus jujuba Mill.) grown in the Loess Plateau. The relationships between plant-based indicators and soil matrix potential as well as meteorological factors of jujube under deficit irrigation compared with well irrigation were determined. The results showed that maximum daily trunk shrinkage increased and maximum daily...

Author(s): Ping Zhang, You-Ke Wang, Jing-Wu Zhan, Xing Wang and Peng Wu

August 2010

Evaluation of substrates from renewable-resources in biosurfactants production by Pseudomonas strains

The aim of the present study is to evaluate biosurfactant production by two strains of Pseudomonas spp. using alternative carbon sources. The bacterial strains used were isolated from the Municipal Park of Mucugê, Bahia State, Brazil. Different vegetable oils and glycerol concentrations were evaluated and used individually, as the sole source of carbon and energy for the production of biosurfactant which...

Author(s): Sidnei Cerqueira dos Santos, Luzimar Gonzaga Fernandez, Juan Carlos Rossi-Alva and Milton Ricardo de Abreu Roque

August 2010

In vitro storage of synthetic seeds: Effect of different storage conditions and intervals on their conversion ability

In vitro derived shoots of olive cv. Moraiolo were employed in synthetic seeds preparation by alginate encapsulation, and then stored in artificial endosperm solution at cold (4°C) and room storage (21 ± 2°C) conditions in interaction with different storage intervals of 0, 15, 30, 45 and 60 days to evaluate the comparative regrowth and conversion capacity of synthetic seeds. Cold stored synthetic...

Author(s): Muhammad Ikhlaq, Ishfaq Ahmad Hafiz, Maurizio Micheli, Touqeer Ahmad, Nadeem Akhtar Abbasi and Alvaro Standardi

August 2010

Structural and metabolic responses of Ceratophyllum demersum to eutrophic conditions

Eutrophication in water bodies affects the growth of aquatic plants. In this study, we conducted static experiments to better understand the structural and metabolic responses of Ceratophyllum demersum under eutrophication conditions. The anatomical structure, nitrogen (N) and phosphorous (P) levels in tissue, malondiadehyde (MDA), and activities of three antioxidases (peroxidase (POD), superoxide...

Author(s): Xiong Hanfeng, Tan Qiling and Hu Chengxiao

August 2010

Effect of Sacoglottis gabonensis and Alstonia boonei on the kinetics of Saccharomyces cerevisiae isolated from palm wine

Sacoglottis gabonensis and Alstonia boonei are botanicals used for the preservation of palm wine in Southern Nigeria. This study investigated the effect of S. gabonensis (0.625%) and A. boonei (0.50%) on the kinetics of Saccharomyces cerevisiae isolated from palm wine (PW). Concentrations of the preservatives used in this study were optimal concentrations of the preservatives that had...

Author(s): A. I. Elijah, P. C. Ojimelukwe, U. S. Ekong and N. U. Asamudo

August 2010

Fermentation characteristics of Korean pear (Pyrus pyrifolia Nakai) puree by the Leuconostoc mesenteroides 51-3 strain isolated from Kimchi

A lactic acid bacterial strain showing fast growth and high acid production when cultured in Korean pear puree was isolated from Kimchi. This strain was analyzed by using the API 50 CHL kit and 16S rRNA sequencing and was thus identified asLeuconostoc mesenteroides 51-3. Korean pear puree was fermented with the L. mesenteroides 51-3 strain at 30°C for 12 h. The changes in pH, titratable acidity...

Author(s): Dong Chung Kim, Hee Jeong Chae, and Man-Jin In

August 2010

The correlation between total antioxidant capacity and nitric oxide concentration in seminal plasma with sperm DNA damage

Sperm DNA quality is important in male fertility. Oxidative stress increases sperm DNA damages. Antioxidants decrease production of free radicals and scavenge them. Nitric oxide (NO) is a free radical which is produced by most cells and has a dual role on cells. Low concentrations of NO is essential in biology and physiology of systems but high level of NO has a detrimental effect on...

Author(s): Nasrin Sheikh, Iraj Amiri, Rezvan Najafi and Mohammad Taghi Goodarzi

August 2010

Cellular energy allocation of pistachio green stink bug, Brachynema germari Kol. (Hemiptera.: Pentatomidae) in relation to juvenoid pyriproxyfen

The purpose of this study is to determine the effects of pyriproxyfen (an insect growth regulator compound) on cellular energy allocation of a heterometabolous insect, Brachynema germari Kol. The fifth instar of this insect was treated with 0, 20, 60, 100, 200 and 500 mg l-1 formulation of pyriproxyfen (Admiral 10EC) and the amount of energy available (Ea), energy consumption (Ec) and cellular energy...

Author(s): Faezeh Bagheri, Khalil Talebi and Vahid Hosseininaveh

August 2010

Refined mapping of loss of heterozygosity in Chinese sporadic gastric carcinoma

The aim of this study is to explore precise deleted regions where the candidate tumor suppressor genes might be located in Chinese sporadic gastric carcinoma. By searching in Genothon, NCBI and GDB databases, 145 polymorphic microsatellite markers were chosen, at a mean density of approximately one marker every 2 - 4 cM, covering 15 chromosomes. These polymorphic microsatellite markers in gastric carcinoma and adjacent...

Author(s): Quan Wang, Yu-Gang Wen, Da-Peng Li, Fei Huang, Chong-Zhi Zhou, Guo-Qiang Qiu, Hua-Mei Tang and Zhi-Hai Peng

August 2010

Antioxidant activity and phenolic compounds from Colchicum luteum Baker (Liliaceae)

The ethanolic extract from corms of the Colchicum luteum Baker (Liliaceae) was investigated phytochemically. During phytochemical studies, compounds 1 - 4 were isolated from the n-butanol fraction. These compounds were identified as colchicines 1, β - Lumicolchicine 2, chlorogenic acid 3 and 3’, 4’, 5.7-Tetrahydroxyflavone 4, on the basis of different modern spectroscopic techniques....

Author(s): Bashir Ahmad

August 2010

Water relations of Pseudomonas solanacearum

The growth of 16 strains of Pseudomonas solanacearum at different water activities was investigated. These studies were undertaken in order to understand the adaptive mechanisms to osmotic stress of bacteria colonizing plants and to elucidate bacterial persistence when the efforts to eradicate disease fail. Due to inadequate growth of some strains on GYS medium, 3 strains, 66, 64 and 46, were selected for...

Author(s): N.T. Mazomba and L.V. Mabinya

August 2010

Increase of hepatic nitric oxide levels in a nutritional model of fatty liver in broiler breeder hens

Fatty liver hemorrhagic syndrome (FLHS) is a metabolic condition of laying hens characterized by the accumulation of excess fat in the liver and liver hemorrhage. It suggested that oxidative damage to the cellular and organelle membranes of the liver increases the susceptibility of the liver to hemorrhage. However, a local chemical component that may affect the incidence of hemorrhage in liver, by affecting the blood...

Author(s): Ardashir Sheikh Ahmadi, Mojtaba Zaghari, Mahmood Shivazad, HosseinHassanpour and Armin Towhidi

August 2010

Correlation between serum lipoproteins and abdominal fat pad in broiler chickens

In this experiment, four hundred day-old broiler chicks were assigned randomly to 16 floor pens with 25 chicks each. Three diets containing 0% fish oil + 7% soybean oil, 3.5% fish oil + 3.5% soybean oil and 7% fish oil + 0% soybean oil and a free oil control diet were formulated. The birds fed fish oil diet showed a lower body weight than control group (P < 0.05). Abdominal fat percent in birds fed soybean oil and...

Author(s): Bahman Navidshad, Hamid Deldar and Gholamreza Pourrahimi