African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the avian infectious bronchitis virus

  Pengju Zhang, Sujun Zhao, Hongning Wang*, Zhiguang Zeng, Lang Feng, Yinglin Liu and Haipeng Cao  
Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, “985 Project” Science Innovative Platform for Resource and Environment Protection of Southwestern, School of Life Science, Sichuan University, Chengdu, 610064, P. R. China.
Email: [email protected]

  •  Accepted: 29 July 2010
  •  Published: 06 December 2010

Abstract

Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the avian infectious bronchitis virus

Abbreviation

Abbreviations: IBV, Infectious bronchitis virus; N, nucleocapsid; M, membrane;YPD, yeast peptone-dextrose; SD, synthetic defined; AD, activation domain; Trp,tryptophan; Leu, leucine; Ade, adenine; OPNG, o-nitrophenyl β-D-galactopyranosideFBS, fetal bovine serum; SDS- PAGE, sodium doedecyl sulfate-polyacrylamide gel electrophoresis; BSA, bovine serum albumi; PBST,phosphate buffered saline with tween 20; ME, membrane endodomain; SARS,severe acute respiratory syndrome.