African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12257

Full Length Research Paper

Evaluation of different methods for total RNA extraction from Thaumastocoris peregrinus

Tarcisio Tomás Cabral de Sousa
  • Tarcisio Tomás Cabral de Sousa
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar
Any Caroliny Pinto Rodrigues
  • Any Caroliny Pinto Rodrigues
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar
Ariadne Marques
  • Ariadne Marques
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar
Felipe Gomes Moreira
  • Felipe Gomes Moreira
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar
Renata Couto Avila
  • Renata Couto Avila
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar
Janaína Fernandes Gonçalves
  • Janaína Fernandes Gonçalves
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar
Farley Souza Ribeiro Menezes
  • Farley Souza Ribeiro Menezes
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar
Marcelo Luiz de Laia
  • Marcelo Luiz de Laia
  • Faculdade de Ciências Agriacutecolas, Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Brazil.
  • Google Scholar


  •  Received: 23 March 2020
  •  Accepted: 27 April 2020
  •  Published: 31 May 2020

Abstract

Thaumastocoris peregrinus is a pest that damages eucalyptus plantations. Recently in Brazil, there are few studies related to its control. With the advancement of molecular biology, several techniques can assist in the discovery of an effective and sustainable control. The different techniques of analysis of gene expression start with the extraction of the total RNA from the material for genomic analysis, hence it is essential that the RNA be intact. Numerous insect RNA extraction protocols are available, but none of them are widely effective, therefore, several factors and intrinsic characteristics of the tissue to be analyzed can affect the quality of the extracted material. Thus, it is necessary to modify the protocols in order to optimize the extraction of RNA. This study aimed to analyze the efficiency of three methods for RNA extraction from T. peregrinus. The results obtained demonstrated that the RNA extracted with only one method was more efficient due to less contamination with DNA and greater integrity of the samples. It should be noted that the determination of an efficient protocol for the extraction of RNA from T. peregrines will assist in future research, which will assist in the discovery of genes present in the extracted RNA sample.
 
Key words: Ribonucleic acid, bronze bug, Eucalyptus.