African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12257

Table of Content: May 2020; 19(5)

May 2020

Morphological and proteomic analyses of Zea mays in response to water stress

Water stress affects plant growth and development, leading to agricultural crop losses in maize cultivation. It also threatens food security in economical crops such as maize, one of the major crops produced worldwide. Transcriptomic studies associated with morphological assessments have been widely conducted on the mechanisms of crop development and stress response; however, data on maize is still very much limited....

Author(s): Precious Thobile Lukhele, Lerato Thamaga, Oziniel Ruzvidzo and Tshegofatso Bridget Dikobe  

May 2020

Morphological and molecular characterisation of Colletotrichum gloeosporioides (Penz) isolates obtained from Dioscorea rotundata (Poir)

Anthracnose disease is a major constraint to yam production in tropical West Africa and anywhere the crop is cultivated. This study determined the cultural characteristics and growth rates of mycelia and also characterised 6 isolates of Colletotrichum gloeosporioides, the causal agent of the yam anthracnose disease, obtained from Dioscorea rotundata leaves, vines and setts in the Tolon District of Ghana. The cultural...

Author(s): Joseph Kwowura Kwodaga, Elias Nortaa Kunedeb Sowley and Benjamin Kongyeli Badii  

May 2020

Evaluation of different methods for total RNA extraction from Thaumastocoris peregrinus

Thaumastocoris peregrinus is a pest that damages eucalyptus plantations. Recently in Brazil, there are few studies related to its control. With the advancement of molecular biology, several techniques can assist in the discovery of an effective and sustainable control. The different techniques of analysis of gene expression start with the extraction of the total RNA from the material for genomic analysis, hence it is...

Author(s): Tarcisio Tomás Cabral de Sousa, Any Caroliny Pinto Rodrigues, Ariadne Marques, Felipe Gomes Moreira, Renata Couto Avila, Janaína Fernandes Gonçalves, Farley Souza Ribeiro Menezes and Marcelo Luiz de Laia

May 2020

HPTLC fingerprint profiles and UPLC-MS identification of potential antioxidant fractions and compounds from Ambrosia maritima L. and Ammi majus L.

This study aimed to develop HPTLC fingerprint profile and identification of antioxidant molecules from active extract and fractions of Ambrosia maritima and Ammi majus using ultra performance liquid chromatography-mass spectroscopy (UPLC-MS). The antioxidant activity evaluated by using DPPH (1,1-diphenyl-2-picryl hydrazyl) method and HPTLC fingerprinting were carried out using CAMAG HPTLC system equipped with...

Author(s): Nazik Salih Mohamed, Mona Salih Mohamed, Ramzi Ahmed Mothana, Wadah Jamal Osman and Hassan Subky Khalid  

May 2020

Diallel analysis for the inheritance study of phytic acid along with morpho-yield traits in bread wheat

In the kernels of wheat, a macro molecule called phytic acid acts as an inhibitor of nutrients. Phytic acid in high concentration is undesirable as it hinders the absoption of other molecules. Regression analysis, the model of additive-dominance and Hotelling’s t2 test were adequate for biological yield, grains spike-1 and phytic acid, while partially adequate results were found for grain yield and 1000-grain...

Author(s): Ijaz Ahmad, Fida Mohammad, Sultan Akber Jadoon, Aurang Zeb, Fazal Munsif and Wiqar Ahmad  

May 2020

Improving peanut protein quality: Expression of a synthetic storage protein

Peanut is an affordable legume used in most households. It represents one of the most important protein supplies worldwide. However, peanut proteins are deficient in several essential amino acids (EAA), like most plant proteins; whereas plants are the main source of dietary proteins consumed by humans and livestock. This could lead to protein malnutrition in areas where people diet relies on one or two staple foods....

Author(s): N’Nan Affoué Sylvie DIBY, Koffi N’Da KONAN, Anthony Okello ANANGA, and Hortense DODO,  

May 2020

Bio-appraisal of three strains of lactobacillus based probiotics on the growth traits and semen characteristics of local toms

The effect of three strains of Lactobacillus based probiotics on the growth performance and semen quality of toms was conducted in a thirty-four week study. Eighty day-old (d 1) poults were randomly divided into 4 groups, replicated twice with 10 birds per replicate and assigned to four treatments; T1 (Lactobacillus delbrueckii subsp Bulgaricus), T2 (Lactobacillus acidophilus), T3 (Lactobacillus sporogens) and T4...

Author(s): Dim C. E., Ekere S. O., Ugwuoke J. I., Ndofor-Foleng H. M. and Onyimonyi A. E.  

May 2020

Comparison of crop production efficiency of compost leachate with chemical fertilizer and evaluating its effect on germination and growth of wheat crop

Increasing public concern in relation to health and environmental issues of organic waste leads to many solutions of waste management. Many different methods are discovered for proper waste management and disposal. Organic fractions of solid waste are treated with aerobic composting. It is one of the better treatments for organic waste. The important issue of concern here is the production of compost leachate with...

Author(s): Ayesha Ameen  

May 2020

Effects of biochar and sewage sludge on spinach (Spinacia oleracea L.) yield and soil NO3- content in texturally different soils in Glen Valley, Botswana

The effects of biochar and sewage sludge application on spinach (Spinacia oleracea L.) yield and soil NO3- content were investigated in typical soils of Botswana (Luvisol, Cambisol) under field conditions. Ten treatments with 3 levels of biochar (0, 2.5, 5 tons ha-1) and sewage sludge (0, 6, 12 ton ha-1) were applied in 2 subsequent seasons. Significant (p < 0.05) yield increase on the Luvisol occurred if sewage...

Author(s): Ugele Majaule, Oagile Dikinya, Baleseng Moseki and Bruno Glaser  

May 2020

Antioxidant, cytotoxic and antimicrobial activities of Dendropanax morbifera and sweet potato extracts for production of health-oriented food materials

The antioxidant, cytotoxic and antimicrobial effects of fermented and non-fermented extracts of ‘the Korean shrub’ Dendropanax morbifera and sweet potato were compared to assess the potential utility of these species in the development of health-oriented food. Non-fermented extract (NFDSE) was obtained from the leaves and branches of D. morbifera and the bodies of sweet potato using distilled water. The...

Author(s): KiBeom Lee, Ju Hyun Park and Yun Sung Kim