African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12210

Full Length Research Paper

Effects of Fusarium verticilloides, its metabolites and neem leaf extract on germination and vigour indices of maize (Zea mays L.)

  Samuel Toba Anjorin1*, Hussaini Anthony Makun2, Titilayo Adesina2 and Ibrahim Kudu3
1Department of Crop Production, Federal University of Technology, Minna, Niger State, Nigeria. 2Department of Biochemistry, Federal University of Technology, Minna, Niger State, Nigeria. 3Department of Microbiology, Federal University of Technology, Minna, Niger State, Nigeria.
Email: [email protected]

  •  Accepted: 13 June 2008
  •  Published: 18 July 2008

Abstract

Maize (Zea mays L.) seed samples were collected from three markets in Minna, Niger State to investigate the effect of Fusarium verticilloides, its metabolites and neem (Azadirachta indica) leaf ethanolic extract on germination of the seeds and seedling vigour. Among the fungi genera isolated from the seeds samples wereAspergillus spp. (41.18%), Fusarium spp. (29.41%) and Rhizopus spp. (23.53%).F. verticilloides metabolite was extracted using dichloromethane and phosphoric acid (10:1) while powdered neem leaf was extracted with ethanol for 72 h. The experiment, which was made up of eight treatments and three replicates, was set up in a germination chamber. At 9 days after sowing (9 DAS), the seeds applied with combined F. verticilloides mycelia and their metabolites had the least germination percentage (30%), the highest rot-index (3.5) and the least seedling vigour (0.93). The seeds applied with neem extract only had the highest germination percentage of 100%, the least rot index (0.5) and seedling vigour index (5.4). Though neem leaf extract proved to be fungitoxic against   F. verticilloides,further investigation on the pathogenic mode of action of fusariotoxin on germination and growth of cereal crops on the field is necessary.

 

Key words: Fusarium verticilloides, metabolites, neem leaf extract, germination, vigour indices, maize.