African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Introduction

Kinetics of exoglucanase and endoglucanase produced by Aspergillus niger NRRL 567

  Mohammad Ishfaq Ghori1, Sibtain Ahmed2,3, Mohammad Aslam Malana1 and Amer Jamil2*  
  1Department of Chemistry, Bahauddin Zakariya University, Multan, Pakistan. 2Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan. 3School of Medicine, University of New Mexico, MSC10-5550, Albuquerque, NM 87131-000, USA.
Email: [email protected]

  •  Accepted: 22 March 2012
  •  Published: 05 April 2012

Abstract

 

In this study we reported for the first time kinetics of exoglucanase (EXG) and endoglucanase (EG) from Aspergillus niger NRRL 567. The optimum pH and temperature for crude EXG and EG was found to be 3.5 and 30°C respectively. Zn2+, Ca2+, Mn2+ and Co2+ enhanced the crude activity of EXG and EG whereas Mg2+, Fe2+ and Hg2+ showed various degree of inhibitory effects. Cu2+ enhanced crude EXG activity and inhibited crude EG activity. The energy of activation (Ea) for the EXG and EG were 21.20 and 22.52 kJ mol-1, respectively. The Q10 values obtained for the EXG and EG were 1.38 and 1.4, respectively. These enzymes had lower Km value that shows their high affinity for the substrates. Overall, the studies demonstrate that these enzymes may be suitable for industrial use.

 

Key words: Exoglucanase, endoglucanase, kinetics, characterization, Aspergillus niger NRRL 567.

Abbreviation

Abbreviations: EXG, Exoglucanase; EG, endoglucanase; Ea, energy of activation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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