April 2012
Phytopharmacology and medicinal properties of Salix aegyptiaca L.
Salix aegyptiaca L. is known as Musk Willow. S. aegyptiaca extracts and essential oils are important areas in drug development with some pharmacological activities in the Middle East, especially in Iran. For a long time S. aegyptiaca has been used in traditional medicines for the relief of anemia and vertigo, as a cardiotonic agent, as well as a fragrance additive in the...
April 2012
Regulation of the flowering time of Arabidopsis thaliana by thylakoid ascorbate peroxidase
Flowering time of higher plants is precisely controlled by various exogenous and endogenous factors. Recent researches implied that H2O2 is a potential flowering initiation factor. In order to confirm this hypothesis, thylakoid ascorbate peroxidase (tAPX) overexpressing Arabidopsis, the mutant line containing a T-DNA insertion and the wild type have been analyzed in this study, since APX was an...
April 2012
Determination of genetic relatedness among selected rice (Oryza sativa, L.) cultivars using microsatellite markers
For plant improvement programs, genetic variation information among different cultivars is very important. Genetic variation among 26 rice (Oryza sativa, L.) accessions, consisting of 13 Iranian and 13 Malaysian cultivars was investigated using microsatellite markers distributed across the rice genome. All the 21 selected microsatellite primers were successfully amplified by polymerase chain reaction...
April 2012
Comparison of the G-174C polymorphism of interleukin (IL)-6 in different countries
Polymorphism of G-174C in the interleukin-6 (IL-6) promoter could affect both the transcription and secretion of IL-6 and may be involved in inflammation related to and the pathogenesis of infectious diseases and chronic diseases. However, IL-6 G-174C polymorphism may differ in various ethnic groups. We recruited 300 Chinese healthy subjects among two ethnic populations in order to assess the nature...
April 2012
Influence of cutting frequency and fertilizer-N application on tiller production and herbage yield distribution over time in a guinea grass (Panicum maximum) sown pasture
An experiment was carried out to evaluate the effects of cutting frequency and nitrogen rates on guinea grass (Panicum maximum) tiller production and herbage yield distribution over time. Plants were grown in the Department of Crop Science Research and Teaching Farm, University of Nigeria, Nsukka. A 4 × 4 factorial experiment replicated three times was set in a randomized complete block design....
April 2012
Expression of hepatitis B surface antigen (HBsAg) gene in transgenic cherry tomato
The plasmid carrying the gene (adr subtype) encoding the hepatitis B surface antigen (HBsAg) was constructed. Cotyledonary leaves of cherry tomato (Lycopersicon esculentum Mill.) were transformed using Agrobacterium tumefaciens strain LBA4404harboring pCAMBIA-1301/HB constructs to express HBsAg. The transformed nature of the plants was confirmed by polymerase chain reaction (PCR)...
April 2012
Characterization and evaluation of Bacillus isolates for their potential plant growth and biocontrol activities against tomato bacterial wilt
About 200 Bacillus isolates were isolated from tomato and potato rhizosphere and examined for their antagonistic activities against Ralstonia solanacearum T-91, the causal agent of tomato bacterial wilt (TBW), in vitro and in vivo. Four strains, AM1, D16, D29 and H8, have shown high potential of antagonistic activity against the pathogen in laboratory and greenhouse...
April 2012
Study of the system of tuberous root induction in vitro from Rehmannia glutinosa
This study investigated the induction system of tuberous root in vitro from Rehmannia glutinosa. The roles of plant growth substance, carbohydrates, and minerals were evaluated for induction and development of tuberous root in vitro. The results show that Murashige and Skoog (MS) contributed greatly to induction of tuberous root in vitro, followed by α-naphthalene acetic...
April 2012
Effects of the nitrogen and phosphorus fertilization on the yield and quality of the hairy vetch (Vicia villosa Roth.) and barley (Hordeum vulgare L.) mixture
This study was intended to determine the effects of nitrogen (0, 30, 60 and 90 kg N ha-1) and phosphorus rates (0, 30, 60, 90 and 120 kg P2O5 ha-1) on dry matter yield, hairy vetch ratio, crude protein yield, acid detergent fiber (ADF), neutral detergent fiber(NDF) and total digestible nutrient (TDN) of hairy vetch-barley mixture (50:50) in Bursa-Turkey in 2009 to 2010 and 2010 to 2011...
April 2012
Cryopreservation and plant regeneration of anther callus in Hevea by vitrification
Callus induced from anther of Hevea brasiliensis was successfully cryopreserved in liquid nitrogen (LN) by vitrification method and subsequently regenerated into plants. The effects of different preculture time, loading and dehydration duration on callusviability after cryopreservation were evaluated. The effective cryopreservation protocol involved preculturing on modified Murashige and Skoog (MS)...
April 2012
Genotypic identification and technological characterization of lactic acid bacteria isolated from traditional Turkish Kargi tulum cheese
Kargi tulum cheese is an artisanal cheese produced through the spontaneous fermentation of raw milk ripened inside a goat-skin bag. The objective of this study was to characterize the dominant species of natural lactic acid bacteria (LAB) found in Kargi tulum cheese. Some technologically important properties of LAB isolates were also determined. Seven samples of cheese of different levels of ripeness were...
April 2012
Kinetics of exoglucanase and endoglucanase produced by Aspergillus niger NRRL 567
In this study we reported for the first time kinetics of exoglucanase (EXG) and endoglucanase (EG) from Aspergillus niger NRRL 567. The optimum pH and temperature for crude EXG and EG was found to be 3.5 and 30°C respectively. Zn2+, Ca2+, Mn2+ and Co2+ enhanced the crude activity of EXG and EG whereas Mg2+, Fe2+ and Hg2+ showed various degree of inhibitory effects....
April 2012
Effect of γ-irradiation on the physicochemical properties of mixed soy protein isolate/starch material
To elucidate the effect of γ-irradiation on the molecular structure and mechanical properties of biodegradable material composed by soybean protein isolated (SPI) and starch, SPI and starch were mixed and modified by succinic anhydride and irradiation. Different doses (0 to 50 kGy) γ-irradiation on the elongation, tensile strength, viscosity, Fourier transform infrared spectroscopy (FTIR),...
April 2012
Effects of different factors on the forward extraction of soy protein in reverse micelle systems
Reverse micelle extraction is a new technology for the extraction of protein. In this research, three kinds of reverse micelle systems, anionic surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micelle system, sodium dodecyl sulfate (SDS) reverse micelle system, and cationic surfactant cetyltrimethyl ammonium bromide (CTAB) reverse micelle system, were used to extract soy protein respectively....
April 2012
Comparative study on calcium, magnesium and cobalt in diabetic and non diabetic patients (males) in Punjab, Pakistan
Minerals are very important because of their important role in the maintenance of human health. Our study was aimed at assessing and comparing the levels of Ca, Mg and Co in the blood samples of diabetic and non diabetic persons (males) of five age groups between one and 75 years. For this purpose, fasting blood samples of diabetic and non diabetic males of selected age groups were collected. The blood...
April 2012
The hymenopterous pollinators of Himalayan foot hills of Pakistan (distributional diversity)
Studies were undertaken to explore the diversity of hymenopterans pollinators from a diverse agro-ecosystems of Himalayan foot hills comprising the orchards of pome and stone fruits at different altitudes from 2200 to 3000 m from sea level. Field experiments were conducted on seven commercial fruit orchards at five various localities. Out of the total 448 specimens, 60.94% were found in an ante-meridian...
April 2012
Ultrastructural study of spermatogenic stages in the protandrous sparid fish Diplodus cervinus cervinus (Lowe, 1838) from the South Eastern Mediterranean coast
The current study was designed to study the ultrastructure of the spermatogenic stages of the protandrous hermaphrodite sparid Diplodus cervinus cervinus. Although, it is a useful tool to enhance understanding of germ cells differentiation in this economic species, none of the available references paid attention to the studied species. The testis of the studied specie is tubular...
April 2012
Detoxification of cyanides in cassava flour by linamarase of Bacillus subtilis KM05 isolated from cassava peel
Defensive cyanogenic glucoside linamarin accounts for 80% cyanide content of cassava and is known to cause severe diseases upon continual consumption. Detoxification of this cyanide would enhance the nutritive quality and hence market value of cassava flour. We isolated cyanogenic glucoside utilizing indigenous bacteria from cyanide rich cassava peel waste and exploited their potential for detoxification....
April 2012
Evaluation of the productive performance characteristics of red tilapia (Oreochromis sp.) injected with shark DNA into skeletal muscles and maintained diets containing different levels of probiotic and amino yeast
This work aimed to study the effect of direct injection of shark (Squalus acanthias L.) DNA into skeletal muscles of red tilapia (Oreochromis sp.) fed at different additive levels (two probiotic levels; 0.3 and 0.5%, two amino yeast levels; 0.5 and 1.0% and a mixed of 0.3% probiotic and 0.5% amino yeast), on the productive performance. The results show that red tilapia injected with DNA had...
April 2012
Effect of gene transfer of Chlorella vulgaris n-3 fatty acid desaturase on mouse breast cancer cells
Chlorella vulgaris had the gene of n-3 fatty acid desaturase (CvFad3) which cansynthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or to convert n-6 to n-3 PUFAs. The objective of this study was to examine whether the CvFad3 gene from C. vulgaris can be functionally expressed in mammalian cells and whether its expression can exert a significant effect on...
April 2012
Selection of ligand peptides with the ability to detect antibodies in enzootic bovine leukosis
Peptides present in phages were selected using phage display technology and immunoassays to find out the antigenic mimetics of immunodominant epitopes of bovine leukosis virus (BLV). The use of antigenic mimetics may result in the enhancement of the sensitivity and specificity of the serologic diagnosis of enzootic bovine leukosis (EBL), contributing directly to disease control. The selections enabled the...
April 2012
Prediction of metabolisable energy of poultry feeds by estimating in vitro organic matter digestibility
The aim of this study was to develop equations to predict the in vivo apparent metabolisable energy (AME) of poultry feeds using an in vitro method for estimation of organic matter digestibility. In this study, a total of 57 samples of feedstuffs and 23 samples complete diets for poultry were used. Dry matter (DM), crude protein (CP), crude fibre (CF), crude fat (CFat)...
April 2012
Identification and characterization of variants in the 5' flanking region of bovine growth hormone gene
Sequence variations within the 5' flanking region of bovine growth hormone gene were identified from six bovine species raised in China. Cloned and sequenced amplified fragments revealed difference in length because of the insertion and deletion mutation. A total of thirty one variation sites were identified in this region within species and among species. Several new single...
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